FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1799149
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCCCCGTACACTTTCGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT47290.2628464902017565No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43290.2406137568372603No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTTCA37170.20659767478958105No Hit
GTCTCTGGTAGCTCCATCAGCAGTGGTGATTACTACTGGAGTTGGATCCG35980.19998343661364343No Hit
GTTATTATACTGCTGACAGTAGTAAATTGCAAAATCTTCAGACTGCAGGCTGGTGATGGTGAGGGTGAACT34850.19370268943817326No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28740.1597421892239053No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27590.15335027838161264No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27450.15257213271385525No Hit
GAGTTCACCCTCACCATCACCAGCCTGCAGTCTGAAGATTTTGCAATTTACTACTGTCAGCAGTATAATAACTGG27170.15101584137834054No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26960.14984862287670447No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25520.14184483886548585No Hit
GTCTGGGACAGAGTTCACCCTCACCATCACCAGCCTGCAGTCTGAAGATT25450.14145576603160717No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24130.13411896402132342No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACC23080.12828287151314316No Hit
CACCTGCACTGTCTCTGGTAGCTCCATCAGCAGTGGTGATTACTACTGGA22710.12622634367692726No Hit
GTAATATACGGCCGTGTCTGCGGCCGTCACAGAGCTCAGCTTCAGGGAGA22290.12389190667365516No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC22050.12255794267178538No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT22020.12239119717155167No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG21820.12127956050332685No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21600.12005676016827957No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC21500.11950094183416715No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21140.1174999958313625No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21080.11716650483089505No Hit
CATCTATGGTGCTTCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCAC20960.11649952282996018No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20400.1133869401589307No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCT20110.11177506699000471No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC19580.10882922981920898No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCGAAAGTGTACGGGGGCCAGTTATTATACTG19460.10816224781827408No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT19290.107217356650283No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGGAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAC19110.10621688364888067No Hit
GTATATTACTGTGCCAGAGCGAAATATTGTCCTAGTGCCAACTGCTATGG19090.10610571998205817No Hit
GTGTTAGCACCAACTTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCTTCCA19020.10571664714817951No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC18860.10482733781359964No Hit
GTCTGTGTCTCCAGGGGGAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTAGCCTGGTA18770.10432710131289848No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18410.10232615531009383No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18140.10082544580799033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25050.045.621851
GTGGTAT6750.043.3468631
GTATCAA63050.041.383441
TATCAAC73200.035.457062
ATCAACG77700.033.5807723
TCAACGC77950.033.5172354
CAACGCA80100.032.6605575
AACGCAG81450.032.076966
CGCGTAT450.004573658630.73451639
TATACCG450.004674513430.5977886
CGCAGAG88350.029.596778
AGAGTAC89200.029.3147411
ACGCAGA90950.028.8271437
GCAGAGT92450.028.3586379
TATTGAG14750.028.22392570
CAGAGTA94500.027.67063110
ACCGATC2850.026.5821127
GTATACG658.7734463E-426.4788511
AGTACGG64700.025.68503413
GAGTACG64700.025.63185512
Produced by FastQC (version 0.11.5)