FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314162
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17290.1315667322597975No Hit
GTAATAGACCGCCGTGTCATCGGCTCTCAGTCTGTTCATTTGCAGATACA16080.12235934382519051No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16030.12197887322871914No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA14880.11322804950987778No Hit
GGTGTATACTACGGAGCCTCTGTGAAGGGCCGTTTCACCAGCTCCAGGGACAACGCCAAGAACTCAGTGTATCTG14610.11117350828893241No Hit
GTCTATTACTGTGTGAGAGATGGCGCCTTCGGATATAGTGGCTACGATCGTGAAGAGTACTGGGGCCAGGGAACC13850.1053903552225677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19750.040.7689441
GTGGTAT7650.040.4819871
GTATCAA45300.037.5999831
TGGTATC8850.034.99292
TATCAAC51600.032.8092352
ATCAACG54900.030.8997753
TCAACGC55550.030.6001514
CAACGCA56250.030.219355
AACGCAG58350.029.0727966
ACGAGAC2850.027.76393722
ACGCAGA63600.026.7188877
CGCAGAG63850.026.5603948
CGTATGC1850.026.19829841
CAGAGTA65650.025.88455610
GCAGAGT66300.025.5789019
CTTATAC3300.025.0252271
AGTACGG43950.024.967813
GAGTACG44550.024.7087512
TCGTATG1701.6189006E-1024.42584440
AGAGTAC70100.024.19231211
Produced by FastQC (version 0.11.5)