FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213585
Sequences flagged as poor quality0
Sequence length20-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC22011.0305030783997005No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12900.6039749982442587No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10340.48411639394152206No Hit
GTATCAACGCAGAGTACGGGTTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGG9020.4223143010979235No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7150.33476133623615895No Hit
TAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTT6870.32165180139054705No Hit
ACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCA5950.27857761546925114No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC5430.25423133647025775No Hit
GTTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGC4980.2331624411826673No Hit
ATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCCCC4930.23082145281737948No Hit
CACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTT4570.21396633658730713No Hit
TTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCC4270.1999204063955802No Hit
GTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC4150.19430203431888943No Hit
CTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGCCTGT3550.16621017393543552No Hit
GGTATCAACGCAGAGTACGGGTTCTAATACGACTCACTATAGGGAAGTGA3530.1652737785893204No Hit
GAGTACGGGTTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCCC3460.16199639487791748No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG3400.15918720883957208No Hit
GTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAA3290.15403703443593886No Hit
TATCAACGCAGAGTACGGGTTCTAATACGACTCACTATAGGGAAGTGATA3180.14888686003230564No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG3180.14888686003230564No Hit
ATATCAACGCAGAGTACGGGGATCTAAAAAAAAAAAAAAAAAAAAAAAAA3150.14748226701313294No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2870.13437273216752113No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2750.12875436009083036No Hit
GTATCAACGCAGAGTACGGGGATCTAAAAAAAAAAAAAAAAAAAAAAAAA2740.1282861624177728No Hit
TCCCTATAGTGAGTCGTATTAGAACCCGTACTCTGCGTTGATAC2710.12688156939860007No Hit
GTATCAACGCAGAGTACGGGTTCTAATACGACTCACTATAGGGA2620.12266779034108201No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2450.1147084298991034No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG2400.11236744153381559No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2340.10955825549547019No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2330.10909005782241263No Hit
GGTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC2230.10440808109183697No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGAGT150.00174173874.43147
CTATCAA150.003287913763.414521
CTCTTAT150.003287913763.414521
ATATCAA1100.063.414521
CAAAACA200.00378639561.1886163
CCGGTTT200.00461496158.20722254
GATGTAC200.00514432756.6327449
GTGGTAT5200.054.877951
AAACAGC200.006069531754.31532745
GTTGGGA200.00749664751.4921440
AGGTACT253.887338E-450.7316132
ATACGGG900.049.3224071
CGGACAT250.00976860148.15293561
ATGGTAT401.4734724E-647.5608941
AAGTACG1200.044.9186171
GGGTCCG357.174066E-444.7977556
AGTGTAC309.558081E-442.2664510
CTTATAC1400.040.7664761
TTATACA1400.038.501672
TATACAC1500.038.0487143
Produced by FastQC (version 0.11.5)