FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2842754
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT128360.4515339702274625No Hit
AACCAGGACAGCCCCCTAAATTGCTCATTTACTGGTCATCTACCCGGGAA85020.2990761775376976No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC80220.2821911428143272No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC79100.2782513013788741No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT77500.27262295647108403No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG75220.2646025649774831No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG71910.2529589264494923No Hit
ATTTTAGACAGGTCCAACAATAAGAACTGCTTAGCTTGGTACCAGCAGAA71320.25088347426474467No Hit
GTTTACTACTGTCAGCAATATTATGGTACTCCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA70190.2469084556736179No Hit
CCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG67680.23807898959952215No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC67250.23656637190555357No Hit
GTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATATTTTAGACAGGTCCAA67020.23575729732505873No Hit
ATATTATGGTACTCCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT66010.23220440460201622No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT61700.2170430505066566No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC61500.2163395073931828No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC59590.20962067065950835No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC56980.20043943302867573No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT56480.1986805752449913No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC54220.19073053806273774No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGCGGCAGTTTA53650.1887254401893375No Hit
CCCCTAAATTGCTCATTTACTGGTCATCTACCCGGGAATCCGGGGTCCCT50590.1779612305531889No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC49530.17423245205177795No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG48310.169940839059588No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGCAGTTCTTATTGTTGGACCTG47230.16614170624682967No Hit
GTCTCCAGAGTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATATTTTAGA42870.15080446637310158No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG42690.1501712775709752No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC42490.14946773445750142No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG42420.14922149436778562No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA41730.14679427062630113No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATATTTTAGACAGGTCCAACAATAAGAACTG41050.1444022240404903No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG40870.14376903523836393No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT40770.14341726368162702No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGCGGCAGTTTACTACTGTCAGCAATATTATGGTACTCCTCAGACGTT40370.14201017745467953No Hit
ATTTAGGGGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGCAGTTCTTATTGTTGGACCTGTCTAAAATATT40110.1410955714071636No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC39510.13898494206674233No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG39460.13880905628837387No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGACCAGTAAATGAGCAATTTAGGGGGCTGTCCTGGTTTCTGCTGGTA39230.13799998170787905No Hit
GGTCCAACAATAAGAACTGCTTAGCTTGGTACCAGCAGAAACCAGGACAGCCCCCTAAATTGCTCATTTACTGGT39160.13775374161816323No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT38860.1366984269479526No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA38600.1357838209004367No Hit
CAATAAGAACTGCTTAGCTTGGTACCAGCAGAAACCAGGACAGCCCCCTAAATTGCTCATTTACTGGTCATCTAC38340.13486921485292078No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGCGGCAGTTTACTACTGTCAGCAATATTATGGTACT37840.13311035706923638No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT37540.13205504239902574No Hit
CCATAATATTGCTGACAGTAGTAAACTGCCGCATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC36320.12776342940683577No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA36160.12720059491605676No Hit
GCTTAGCTTGGTACCAGCAGAAACCAGGACAGCCCCCTAAATTGCTCATT35380.12445677677350905No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGAGTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG35070.12336628494762474No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG34850.12259238752280359No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC33920.11932091204515058No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33750.11872290039869789No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG33640.11833595168628731No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT33600.11819524306359255No Hit
CGTCTGAGGAGTACCATAATATTGCTGACAGTAGTAAACTGCCGCATCTT33300.1171399283933819No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA32750.11520518483132905No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA32670.11492376758593956No Hit
GTAGTAAACTGCCGCATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA32640.11481823611891849No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG32410.11400916153842366No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGAGTCCCTGGCTGTGTCTCTGGGCGAGAGGG32390.11393880722707628No Hit
TTATGGTACTCCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT32220.11334079558062357No Hit
TTACTACTGTCAGCAATATTATGGTACTCCTCAGACGTTCGGCCAAGGGA31780.11179300073098129No Hit
CTCCAGAGTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGC31480.11073768606077064No Hit
ACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC30800.10834563947495984No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA30480.10721997049340183No Hit
CTCCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC30220.10630536444588594No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC30140.10602394720049642No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC29920.10525004977567527No Hit
TCTGAGGAGTACCATAATATTGCTGACAGTAGTAAACTGCCGCATCTTCA29030.10211928292071701No Hit
GTACCATAATATTGCTGACAGTAGTAAACTGCCGCATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT28610.10064184238242212No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)