FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2842754
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT124760.4388701941849348No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT80200.28212078850297984No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC75600.26593929689308327No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG71610.25190361177928167No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC67930.23895841849136434No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC67130.23614424603746928No Hit
GTAGTAAACTGCCGCATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA65600.230762141219395No Hit
GTTTACTACTGTCAGCAATATTATGGTACTCCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA65320.22977718086053173No Hit
AACCAGGACAGCCCCCTAAATTGCTCATTTACTGGTCATCTACCCGGGAA65040.22879222050166845No Hit
ATATTATGGTACTCCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT63630.2238322415516784No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT63460.22323422990522568No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63280.2226010411030993No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC62310.21918885700275156No Hit
CCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG60490.21278661467014026No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC58780.2067713210499396No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC58690.2064547266488764No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT56070.1972383118623701No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC55150.19400201354039076No Hit
ATTTTAGACAGGTCCAACAATAAGAACTGCTTAGCTTGGTACCAGCAGAA54940.1932632932712433No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC49800.17518223525496754No Hit
CCATAATATTGCTGACAGTAGTAAACTGCCGCATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC49590.1744435149858201No Hit
GTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATATTTTAGACAGGTCCAA48260.16976495328121954No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG47550.1672673752283877No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGCGGCAGTTTA47310.16642312349221916No Hit
CGTCTGAGGAGTACCATAATATTGCTGACAGTAGTAAACTGCCGCATCTT44430.15629210265819693No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA44290.15579962247876533No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG43890.15439253625181779No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC43380.15259850131245967No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA42520.1495732659245225No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT41720.14675909347062743No Hit
GTACCATAATATTGCTGACAGTAGTAAACTGCCGCATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT40170.14130663434120574No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG39910.14039202829368985No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT39840.14014578820397403No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG39800.14000507958127928No Hit
CCCCTAAATTGCTCATTTACTGGTCATCTACCCGGGAATCCGGGGTCCCT39400.13859799335433176No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT38330.1348340376972471No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG38080.13395460880540488No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGCGGCAGTTTACTACTGTCAGCAATATTATGGTACTCCTCAGACGTT36950.12997959021427813No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA36340.12783378371818316No Hit
TCTGAGGAGTACCATAATATTGCTGACAGTAGTAAACTGCCGCATCTTCA34850.12259238752280359No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG33950.11942644351217165No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG33720.11861736893167682No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT33710.11858219177600313No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGCGGCAGTTTACTACTGTCAGCAATATTATGGTACT33660.1184063059976347No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA33590.11816006590791887No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT33540.11798418012955043No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGACCAGTAAATGAGCAATTTAGGGGGCTGTCCTGGTTTCTGCTGGTA33470.11773794003983461No Hit
ATTTAGGGGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGCAGTTCTTATTGTTGGACCTGTCTAAAATATT33370.11738616848309773No Hit
GTCTCCAGAGTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATATTTTAGA32460.11418504731679209No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGCAGTTCTTATTGTTGGACCTG32000.11256689815580244No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC31910.11225030375473924No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG31680.11144122917424441No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30960.10890847396573887No Hit
TTATGGTACTCCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT30680.10792351360687559No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATATTTTAGACAGGTCCAACAATAAGAACTG30440.10707926187070707No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT30140.10602394720049642No Hit
CAGTAGTAAACTGCCGCATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA29740.1046168609735489No Hit
GGTCCAACAATAAGAACTGCTTAGCTTGGTACCAGCAGAAACCAGGACAGCCCCCTAAATTGCTCATTTACTGGT29630.10422991226113833No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC29500.10377260923738038No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29370.10331530621362243No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC29260.10292835750121186No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28610.10064184238242212No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT28540.10039560229270629No Hit
TTACTACTGTCAGCAATATTATGGTACTCCTCAGACGTTCGGCCAAGGGA28520.10032524798135893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT12750.049.6005171
TGGTATC14050.044.7381252
GGTATCA38750.041.9534761
GTATCAA96450.040.481321
TATCAAC110800.035.2781262
TCAACGC117400.033.3140534
ATCAACG117500.033.309733
CAACGCA119850.032.820715
AACGCAG122450.032.2912946
CGCAGAG132000.029.8771298
ACGCAGA132600.029.690217
AGAGTAC133500.029.36109511
GCAGAGT135300.028.9963389
CAGAGTA135950.028.90815410
AGTACGG90950.026.54793513
GAGTACG91350.026.46597312
TTAACGG2500.024.81897535
GAGTACT61850.024.00797312
AGTACTT64450.022.93586513
GTACGGG106950.022.38782114
Produced by FastQC (version 0.11.5)