FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1262023
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54950.4354120329027284No Hit
GATCTATGCTGTGTCCACTTTGCAAAGTGGGGTCCCATCAAGATTCAGTGGCAGTGGATATGGGACAGATTTCA45160.3578381693519056No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG40170.3182984779199745No Hit
CTGATACTCTGATGACAGTAATAAATTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC37550.2975381589717462No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGTT36630.2902482759822919No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36400.28842580523492833No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35950.2848601015987823No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33830.26806167557960514No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32330.25617599679245145No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31020.24579583731833732No Hit
GAGTATCAGTATCCCGTACACTTTTGCCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT29870.23668348358151953No Hit
GGATACTGATACTCTGATGACAGTAATAAATTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT29550.23414787210692672No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCTACTCTGG29470.23351396923827855No Hit
TCAGTATCCCGTACACTTTTGCCCAGGGGACCAAGCTGGAGATCAAACGA28630.22685798911747249No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27890.22099438758247672No Hit
GTCTCTGGTGGCTCCATCAGTAGTACTAATTACTATTGGGACTGGATCCG27360.2167947810776824No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGGCAAAAGTG27300.21631935392619628No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27150.2151307860474809No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCT27080.21457612103741372No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT26440.2095048980882282No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC25000.19809464645256067No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24250.19215180705898388No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC23010.18232631259493687No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22730.18010765255466818No Hit
GTCTGCATCTGTAGGAGACAAAGTCACCATCGCTTGCCGGGCAAGTCAGA21840.173055483140957No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCC21390.16948977950481092No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21250.1683804494846766No Hit
GCTCCTGGGACTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCGTCCTCC20330.16109056649522235No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC20070.1590303821721157No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACT20040.15879266859637264No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19990.15839647930346754No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT19840.15720791142475216No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG19800.15689095999042807No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19670.15586086782887476No Hit
GTCTCCGTCCTCCCTGTCTGCATCTGTAGGAGACAAAGTCACCATCGCTTGCCGGGCAAGTCAGAGCGTTAACAC19650.15570239211171272No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT19240.15245363990989072No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19080.15118583417259432No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18820.1491256498494877No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18750.14857098483942052No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGGCAAAAGTGTACGGGATA18570.14714470338496208No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18520.14674851409205697No Hit
GGGCAAAAGTGTACGGGATACTGATACTCTGATGACAGTAATAAATTGCA18430.14603537336482775No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18410.1458768976476657No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA18180.14405442690030215No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTC18040.1429450968801678No Hit
GTCTATTACTGTGCGAGACATCTCGCCGGTTTGGTTCGGGGAGTTACTCA17990.14254890758726269No Hit
GTAATAGACGGCCGTGTCTGCGGCGGTCGCAGACCTTAGTTTCAGGGAGA17810.14112262613280424No Hit
CTTTTGCCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA17180.1361306410421997No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16880.13375350528476898No Hit
TCTATGCTGTGTCCACTTTGCAAAGTGGGGTCCCATCAAGATTCAGTGGCAGTGGATATGGGACAGATTTCACT16810.1331988402747018No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16280.12899923376990752No Hit
CTCCTGATCTATGCTGTGTCCACTTTGCAAAGTGGGGTCCCATCAAGATT16270.1289199959113265No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16060.127256000881125No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGTTTAAAT15830.12543353013376143No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15780.1250373408408563No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15710.12448267583078915No Hit
ATGCTGTGTCCACTTTGCAAAGTGGGGTCCCATCAAGATTCAGTGGCAGTGGATATGGGACAGATTTCACTCTCA15570.1233733458106548No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCGTCCTCCCTGTCTGCATCTGTAGGAGACAAAGTCACCATCG15190.12036230718457587No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15080.1194906907401846No Hit
CTCTGAGAGTCATGGATCTCATGTGCAAGAAAATGAAGCTCCTGTGGTTC14870.1178266957099831No Hit
GGATATGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGA14760.11695507926559182No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14700.11647965211410569No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT14670.11624193853836261No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCTACTCTGGCTCC14530.11513260851822828No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAATTTATTACTGTCATCAGAGTATCAGTATC14510.11497413280106622No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14490.11481565708390418No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14340.11362708920518881No Hit
CAGCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGTTTAAATAGGTGTTAACGCTCTGA14170.1122800456093114No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA14060.11140842916492014No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAATTTATTACTGTCATC14010.111012239872015No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13910.11021986128620477No Hit
GAGTGAAATCTGTCCCATATCCACTGCCACTGAATCTTGATGGGACCCCA13870.10990290985188067No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13730.10879357983174633No Hit
ATATGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATT13670.10831815268026018No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13580.10760501195303097No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCATATCCACTGCCACTGAATCTTGATGGGACCCCACTTTGCAAAGTGGA13550.10736729837728791No Hit
GTCCCATATCCACTGCCACTGAATCTTGATGGGACCCCACTTTGCAAAGT13200.10459397332695204No Hit
GTATCAACGCAGAGTACGGGGAGTCAGTCTCAGTCAGGACACAGCATGGA13140.10411854617546591No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13120.10396007045830385No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC13120.10396007045830385No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCATATCCACTGCCACTGAATCT13000.10300921615533157No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12700.10063208039790084No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12680.10047360468073878No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12660.10031512896357672No Hit
GTAGGAGACAAAGTCACCATCGCTTGCCGGGCAAGTCAGAGCGTTAACACCTATTTAAACTGGTATCAGCAGAAA12650.10023589110499571No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12630.10007741538783366No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT150.002336975769.1382439
GTGGTAT3650.055.5200231
CGCTAAT1650.045.9546632
TCGCTAA1750.045.2675531
AACGGAT6750.043.03838770
CGTATGC1850.043.02760341
ATGCCGT1900.041.9707344
ACGAGAC2300.041.8354622
GTATCAA39600.041.199211
TCGTATG1700.040.69552640
TCTTATA3100.039.883832
GTAGATC350.001373859639.2756623
ATCTCCG2300.038.80873510
TGCCGTC2150.037.1022945
CTCGCTA2150.036.8339730
CTTATAC2450.036.450021
TATCAAC45050.036.2884332
CTCTTAT3550.035.798421
TATACAC2700.035.616433
ACACATC2800.035.5709956
Produced by FastQC (version 0.11.5)