FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1262023
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50100.3969816714909316No Hit
GATCTATGCTGTGTCCACTTTGCAAAGTGGGGTCCCATCAAGATTCAGTGGCAGTGGATATGGGACAGATTTCA41020.3250336958993616No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG39860.31584210430396276No Hit
CTGATACTCTGATGACAGTAATAAATTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC37120.2941309310527621No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33870.2683786270139292No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGTT32860.2603756032972458No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31250.24761830806570087No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30080.23834747861172104No Hit
GAGTATCAGTATCCCGTACACTTTTGCCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT30050.23810976503597794No Hit
GTCTCTGGTGGCTCCATCAGTAGTACTAATTACTATTGGGACTGGATCCG28370.2247978047943659No Hit
GGATACTGATACTCTGATGACAGTAATAAATTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT28360.22471856693578482No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT28320.22440161550146076No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27360.2167947810776824No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27300.21631935392619628No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGGCAAAAGTG27010.21402145602734657No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCTACTCTGG25740.2039582479875565No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25490.20197730152303087No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25420.20142263651296372No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCT25320.20063025792715344No Hit
TCAGTATCCCGTACACTTTTGCCCAGGGGACCAAGCTGGAGATCAAACGA24900.19730226786675045No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24810.19658912713952123No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC24740.19603446212945408No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23530.18644668124115013No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT23460.18589201623108295No Hit
GTCTGCATCTGTAGGAGACAAAGTCACCATCGCTTGCCGGGCAAGTCAGA22910.18153393400912662No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22370.17725508964575132No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22360.17717585178717027No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC21690.17186691526224165No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21440.16988596879771606No Hit
GTAATAGACGGCCGTGTCTGCGGCGGTCGCAGACCTTAGTTTCAGGGAGA20530.16267532366684284No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20260.16053590148515517No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCC19930.15792105215198138No Hit
GTCTATTACTGTGCGAGACATCTCGCCGGTTTGGTTCGGGGAGTTACTCA19080.15118583417259432No Hit
GGGCAAAAGTGTACGGGATACTGATACTCTGATGACAGTAATAAATTGCA18530.146827751950638No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18310.14508451906185543No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18290.1449260433446934No Hit
GTCTCCGTCCTCCCTGTCTGCATCTGTAGGAGACAAAGTCACCATCGCTTGCCGGGCAAGTCAGAGCGTTAACAC17860.14151881542570935No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17540.13898320395111657No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17460.13834930108246837No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACT17360.13755692249665816No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17090.1354175003149705No Hit
GCTCCTGGGACTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCGTCCTCC16970.1344666460119982No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16770.13288188884037772No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGGCAAAAGTGTACGGGATA16550.13113865595159516No Hit
CTTTTGCCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA16530.13098018023443314No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16390.1298708502142988No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16080.12741447659828703No Hit
CTCCTGATCTATGCTGTGTCCACTTTGCAAAGTGGGGTCCCATCAAGATT15700.12440343797220813No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15620.12376953510355992No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15570.1233733458106548No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA15260.12091697219464305No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTC15150.12004535575025178No Hit
ATGCTGTGTCCACTTTGCAAAGTGGGGTCCCATCAAGATTCAGTGGCAGTGGATATGGGACAGATTTCACTCTCA15100.11964916645734666No Hit
GGATATGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGA15030.11909450144727948No Hit
TCTATGCTGTGTCCACTTTGCAAAGTGGGGTCCCATCAAGATTCAGTGGCAGTGGATATGGGACAGATTTCACT15020.11901526358869846No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCGTCCTCCCTGTCTGCATCTGTAGGAGACAAAGTCACCATCG14950.11846059857863128No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14900.11806440928572619No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGTTTAAAT14830.117509744275659No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14580.1155287978111334No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA14520.11505337065964726No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14480.11473641922532314No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14180.11235928346789241No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14000.11093300201343399No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCTACTCTGGCTCC13890.11006138556904273No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13880.1099821477104617No Hit
GTCCCATATCCACTGCCACTGAATCTTGATGGGACCCCACTTTGCAAAGT13850.10974443413471861No Hit
GAGTGAAATCTGTCCCATATCCACTGCCACTGAATCTTGATGGGACCCCA13820.10950672055897555No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAATTTATTACTGTCATCAGAGTATCAGTATC13760.1090312934074894No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCATATCCACTGCCACTGAATCT13620.10792196338735506No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAATTTATTACTGTCATC13610.10784272552877405No Hit
CTCTGAGAGTCATGGATCTCATGTGCAAGAAAATGAAGCTCCTGTGGTTC13570.10752577409444994No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13530.10720882266012585No Hit
GTGGGGTCCCATCAAGATTCAGTGGCAGTGGATATGGGACAGATTTCACT13500.10697110908438277No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13240.10491092476127614No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13240.10491092476127614No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCATATCCACTGCCACTGAATCTTGATGGGACCCCACTTTGCAAAGTGGA13230.10483168690269512No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT13020.1031676918724936No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13000.10300921615533157No Hit
ATATGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATT12920.10237531328668337No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12720.10079055611506287No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)