FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297948
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3460.11612764643494837No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCGC150.002250337569.7866154
CGATTGT300.003569797462.10523270
CGTTTGC200.00709292552.22461349
GGTATCA4600.045.7354131
GTATCAA13900.045.406381
TATCAAC16800.037.3630872
ATCAACG17550.035.569853
TCAACGC17800.035.0702744
CAACGCA17900.034.874355
AACGCAG18400.033.9266786
ACGCAGA19550.031.9202737
CGCAGAG19700.031.846898
AGTCGCC450.004360814631.02681455
AGAGTAC20650.030.54871411
CAGAGTA21000.030.20371610
GCAGAGT21300.029.6164769
CGTAGAG703.8543265E-529.55703728
TGGTATC1301.9645086E-1029.1832
CGCGGTA854.2039555E-628.58994543
CACCGCT500.007438144627.81540546
Produced by FastQC (version 0.11.5)