FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756238

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756238
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297948
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3990.1339159853397237No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTAGG252.5721235E-455.1650162
GGTATCA5850.046.5915641
GTGGTAT2150.043.3272741
CACTAAT609.100714E-442.68311770
TGGTATC2200.040.7468872
GTATCAA15300.040.3643231
TATTCTA700.001941686136.5855370
TATCAAC17100.036.2927742
ATCAACG18050.033.9720343
TCAACGC18150.033.762174
CAACGCA18600.033.1137435
TAACGGC751.6523154E-632.2283836
AACGCAG19300.032.091016
ACGCAGA19850.031.0284947
TAGGCTA450.00460495730.68335535
GTCCTAG450.00461635130.6678661
CGCAGAG19900.030.6047178
CCGTTAA450.004665981530.60091616
GCCGTTA450.004669816730.59577815
TACCGCG450.004677494530.5855088
Produced by FastQC (version 0.11.5)