FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences868638
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10340.11903692907747532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18050.040.6835671
GTATCAA42850.039.2398871
GTGGTAT6150.034.593861
AATCGCG501.8535706E-434.52360536
TATCAAC49500.033.825722
ATCAACG51550.032.414013
TCAACGC51550.032.1477744
CTCGTAT652.264668E-531.96051839
CAACGCA52200.031.8131985
AACGCAG54200.030.7025836
TAAGGCG2250.030.55313329
ACGTTAC450.0047493430.49684516
ACGAGAC2300.029.83730722
TGGTATC7100.029.4784012
ACGCAGA57750.028.8696637
CGCAGAG58600.028.3338268
AGAGTAC59700.027.75429711
CTAAACG756.8015576E-427.65541369
CAGAGTA61250.027.05194310
GCAGAGT61600.026.8982419
Produced by FastQC (version 0.11.5)