FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500350
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC31200.6235635055461177No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20820.4161087238932747No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC18840.37653642450284797No Hit
GTATAAAACTTACCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT18420.3681423003897272No Hit
GATCTATGCTGCATCCAGTTTGCAGAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCA15620.31218147296892174No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13000.2598181273108824No Hit
GTATCAACGCAGAGTACGGGAATCAGACCCAGTCAGGACACAGCATGGAC12860.2570200859398421No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12790.255621065254322No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12490.24962526231637855No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11800.2358349155591086No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11770.2352353352653143No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG11570.23123813330668533No Hit
GCATAGATCAGGGACCTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGC10990.21964624762666135No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10880.2174477865494154No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT10750.2148496052763066No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGTCAGATGTGACAT10660.21305086439492357No Hit
GTAGGAGACAGACTCACCATCAGTTGTCGGGCGAGTCAGGCCATTAGCAATTATGTCGCCTGGTTTCAGCAGAAA10480.2094533826321575No Hit
GTCTGCATCTGTAGGAGACAGACTCACCATCAGTTGTCGGGCGAGTCAGG10370.20725492155491157No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10220.20425702008593985No Hit
GTCTCCATCCTCACTGTCTGCATCTGTAGGAGACAGACTCACCATCAGTT10020.2002598181273109No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT9880.19746177675627063No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCC9850.19686219646247627No Hit
GTATCAACGCAGAGTACGGGTAAGTTTTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTG9620.19226541421005297No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9110.1820725492155491No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC9080.18147296892175477No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8890.17767562706105725No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG8860.17707604676726293No Hit
GTTTCCCAGGTGTCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCACTGTCTGCATCTGTAGGAGACAGA8800.17587688617967423No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA8800.17587688617967423No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8580.17147996402518237No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8510.17008094333966223No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCC8280.16548416108723893No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC8040.1606875187368842No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8030.16048765863895273No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC7940.1586889177575697No Hit
GGCCAAAAGTGTACGGGTAAGTTTTATACTGTTGGCAGTAATAAGTTGCA7740.15469171579894075No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG7660.15309283501548915No Hit
GTAATACGAAGCCGTGTCCGCGGCGGTCACAGAGATCACTTTCAAGTAGA7630.1524932547216948No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT7390.14769661237134005No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT7300.14589787148995703No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGCCAACAGTATAAAACTTAC7270.1452982911961627No Hit
TCGTATTACTGTGCGAGAGGCTTAGGTGTGGGAGTGGGAGCTTCCACATGGGGCCAGGGAACCCTGGTCACCGT7250.1448985710002998No Hit
AGTCAGGCCATTAGCAATTATGTCGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCTAGGTCCCTGATCTATG7250.1448985710002998No Hit
ATGCTGCATCCAGTTTGCAGAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCA7030.14050164884580793No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6990.13970220845408213No Hit
TCCTCACTGTCTGCATCTGTAGGAGACAGACTCACCATCAGTTGTCGGGC6940.1387029079644249No Hit
GTCCCTGATCTATGCTGCATCCAGTTTGCAGAGTGGGGTCCCATCAAAGT6720.13430598580993305No Hit
GCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGTCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCA6550.13090836414509843No Hit
GAATCAGACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTT6480.1295093434595783No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6210.12411312081542919No Hit
CAGTAATACGAAGCCGTGTCCGCGGCGGTCACAGAGATCACTTTCAAGTAGAAGTGGTTCTTGGACGTGTCTG6190.12371340061956632No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6010.12011591885680024No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG5930.11851703807334865No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC5920.1183171779754172No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT5860.11711801738782852No Hit
GTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCACT5840.11671829719196562No Hit
CACCATCAGTTGTCGGGCGAGTCAGGCCATTAGCAATTATGTCGCCTGGTTTCAGCAGAAACCAGGGAAAGCCC5800.11591885680023983No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA5800.11591885680023983No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA5770.11531927650644548No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA5760.11511941640851403No Hit
GTCATGGACCTCCTGCACAAGAACATGAAAAACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC5730.1145198361147197No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT5660.11312081542919955No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGCCAAC5650.11292095533126811No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCG5600.11192165484161087No Hit
CCATCCTCACTGTCTGCATCTGTAGGAGACAGACTCACCATCAGTTGTCG5420.10832417307884482No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA5380.10752473268711901No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA5370.10732487258918756No Hit
GAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGC5350.10692515239332466No Hit
CCTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGCGACATAATTGCTAA5350.10692515239332466No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA5270.10532627160987308No Hit
ATGCAGCATAGATCAGGGACCTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGCGACATAATTGCTAATGGC5260.10512641151194164No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT5260.10512641151194164No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA5240.10472669131607874No Hit
TCTACAACCCGTCCCTCAACAGTCGAGTCACCATATCAGCAGACACGTCC5230.10452683121814729No Hit
GATCAGGGACCTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGCGACAT5230.10452683121814729No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG5230.10452683121814729No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA5150.10292795043469571No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT5110.10212851004296992No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC5040.10072948935744977No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC5030.10052962925951835No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG5010.10012990906365545No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGGT205.6382053E-498.8852970
GTGGTAT1750.049.1731951
TGGTATC1800.047.8072782
GGTATCA5850.046.483381
GTATCAA18150.045.705341
CGTCAGA551.4941543E-743.808855
TATCAAC20700.040.4075432
GGGATTC451.0137174E-438.24964518
ATCAACG22000.038.019823
TCAACGC22150.037.7623524
CAACGCA22850.036.90685
AACGCAG22950.036.895976
ACGGGAT1550.035.5280516
GACCCAT750.002362828735.15921470
CAGTTCG7650.034.46981470
CGGGATT709.889209E-734.4246817
CGCAGAG25350.033.531948
ACGCAGA25700.033.0752837
AGAGTAC26000.032.6936411
CAGAGTA26150.032.50610710
Produced by FastQC (version 0.11.5)