FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500350
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC39830.7960427700609572No Hit
GTATAAAACTTACCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT19380.3873288697911462No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18240.36454481862696114No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC15080.30138902768062353No Hit
GATCTATGCTGCATCCAGTTTGCAGAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCA14800.295792944938543No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12890.2576196662336364No Hit
GTATCAACGCAGAGTACGGGAATCAGACCCAGTCAGGACACAGCATGGAC11980.23943239732187468No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11940.2386329569301489No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11550.23083841311082243No Hit
GTATCAACGCAGAGTACGGGTAAGTTTTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTG11410.22804037173978214No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11320.2262416308583991No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11300.22584191066253623No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10490.20965324273008895No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA10030.20045967822524235No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9950.19886079744179075No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG9490.18966723293694412No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT9320.18626961127210953No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC9160.18307184970520637No Hit
GCATAGATCAGGGACCTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGC8720.17427800539622262No Hit
GGCCAAAAGTGTACGGGTAAGTTTTATACTGTTGGCAGTAATAAGTTGCA8690.1736784251024283No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG8600.17187968422104527No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGTCAGATGTGACAT8450.16888178275207355No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT8380.1674827620665534No Hit
GTCTCCATCCTCACTGTCTGCATCTGTAGGAGACAGACTCACCATCAGTT8330.16648346157689617No Hit
GTAGGAGACAGACTCACCATCAGTTGTCGGGCGAGTCAGGCCATTAGCAATTATGTCGCCTGGTTTCAGCAGAAA8310.1660837413810333No Hit
GTCTGCATCTGTAGGAGACAGACTCACCATCAGTTGTCGGGCGAGTCAGG8310.1660837413810333No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8150.1628859798141301No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8070.16128709903067853No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCC8070.16128709903067853No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8050.16088737883481563No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT7720.15429199560307785No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT7190.14369941041271111No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC6940.1387029079644249No Hit
CTGCAAACTGGATGCAGCATAGATCAGGGACCTAGGGGCTTTCCCTGGTT6880.1375037473768362No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCG6770.1353052862995903No Hit
TCGTATTACTGTGCGAGAGGCTTAGGTGTGGGAGTGGGAGCTTCCACATGGGGCCAGGGAACCCTGGTCACCGT6740.13470570600579596No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACTTTGATGGGACCCCA6730.1345058459078645No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6720.13430598580993305No Hit
GTCCCTGATCTATGCTGCATCCAGTTTGCAGAGTGGGGTCCCATCAAAGT6680.13350654541820725No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT6620.13230738483061857No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6610.1321075247326871No Hit
ATGCTGCATCCAGTTTGCAGAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCA6580.13150794443889277No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGCCAACAGTATAAAACTTAC6580.13150794443889277No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCC6490.12970920355750976No Hit
GTAATACGAAGCCGTGTCCGCGGCGGTCACAGAGATCACTTTCAAGTAGA6450.12890976316578395No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6430.12851004296992105No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA6380.1275107424802638No Hit
GTTTCCCAGGTGTCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCACTGTCTGCATCTGTAGGAGACAGA6260.12511242130508643No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC6160.12311382032577196No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG6080.12151493954232037No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6050.12091535924852602No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA5930.11851703807334865No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGCCAAC5910.11811731787748575No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA5830.11651843709403417No Hit
GTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCACT5800.11591885680023983No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT5800.11591885680023983No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT5780.11551913660437693No Hit
AGTCAGGCCATTAGCAATTATGTCGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCTAGGTCCCTGATCTATG5700.11392025582092535No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC5600.11192165484161087No Hit
GCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGTCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCA5580.11152193464574799No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG5540.11072249425402218No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA5530.11052263415609073No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG5520.11032277405815928No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA5500.10992305386229641No Hit
ACTTATTACTGCCAACAGTATAAAACTTACCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA5470.10932347356850206No Hit
GTCATGGACCTCCTGCACAAGAACATGAAAAACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC5450.10892375337263915No Hit
TCCTCACTGTCTGCATCTGTAGGAGACAGACTCACCATCAGTTGTCGGGC5430.10852403317677625No Hit
GTCCCAGATCCACTGCCGCTGAACTTTGATGGGACCCCACTCTGCAAACT5360.10712501249125611No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG5260.10512641151194164No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA5190.10372739082642152No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG5100.10192864994503847No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT5080.10152892974917557No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT5070.10132906965124412No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAC200.00739888351.67503734
TTTATCG200.00743417751.61288530
GGTATCA6050.050.592241
GTGGTAT1250.049.5235521
GTATCAA19250.046.629321
TATCAAC21400.041.589852
TGGTATC1550.039.9063642
TTCGGGG4100.039.66697370
CGGGATC801.713488E-938.6322217
ATCAACG23150.038.4228323
TCAACGC23200.038.3093644
CGGGAAC451.0285681E-438.1552817
AACGCAG23800.037.4802746
CAACGCA23750.037.418465
GCTTGTC655.5791315E-737.0368421
GCTTGTA655.5791315E-737.0368421
CGCAGAG25400.034.9842348
GCTCGTA400.002465112634.86285455
AGAGTAC26150.033.98086511
CAGAGTA26500.033.79099710
Produced by FastQC (version 0.11.5)