FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1470309
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT65850.4478650406139118No Hit
GTATTACTGTCAGCAGTACCATGACTCGTTCACTTTTGGCCAGGGGACCCAACTGGAGATGAAACGAACTGTGG63260.4302496958122408No Hit
CATCTATAGTGCATCCATCAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA55380.3766555193500142No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA50200.3414248297466723No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA43700.29721643545676457No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41740.28388590425550003No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTA41320.2810293618552291No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT39550.2689910760255157No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38970.2650463269965701No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38460.26157766836766966No Hit
CTCCTGCAGGGCCAGCCAGAGTGTCAATACCAATTTCTTAACCTGGTACC37870.25756490642443186No Hit
CTATAGATGAGGAGCCTGGGAGCCTGGCCACGTTTGTGTTGGTACCAGGTTAAGAAATTGGTATTGACACTCTGG34240.23287621853637566No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33830.2300876890503969No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT33760.22961159865035174No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGCCAGAGTGTCAATAC32960.22417056550697845No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGC32830.2232863976211803No Hit
GTGTATTACTGTCAGCAGTACCATGACTCGTTCACTTTTGGCCAGGGGAC32030.21784536447780703No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28710.195265076932808No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC28540.19410885738984118No Hit
GTGCAGCCACAGTTCGTTTCATCTCCAGTTGGGTCCCCTGGCCAAAAGTG28310.1925445603611214No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27160.1847230752175223No Hit
ACCATGACTCGTTCACTTTTGGCCAGGGGACCCAACTGGAGATGAAACGA26110.1775817192168449No Hit
TCTATAGTGCATCCATCAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT25140.1709844665305048No Hit
GTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGCCAGA24890.16928414367320066No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTGAT24580.16717574333014354No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA24260.16499933007279424No Hit
GTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCAC24160.16431920092987257No Hit
AGTCATGGTACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGT23850.16221080058681542No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23800.1618707360153546No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23660.16091855521526427No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT23580.16037445190092695No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATCTCCAGTTGGGTCC22760.15479739292896935No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22570.1535051475574182No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTA21990.14956039852847258No Hit
CCTCATCTATAGTGCATCCATCAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTT20380.1386103193274339No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19890.13527768652711775No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19520.13276120869830763No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19370.13174101498392515No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18730.12738818846922653No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18710.1272521626406422No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18590.1264360076691362No Hit
GTACCATGACTCGTTCACTTTTGGCCAGGGGACCCAACTGGAGATGAAACGAACTGTGGCTGCACCATCTGTCTT18440.12541581395475374No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18420.1252797881261694No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18390.1250757493832929No Hit
ACACAAACGTGGCCAGGCTCCCAGGCTCCTCATCTATAGTGCATCCATCAGGGCCACTGGCGTCCCAGACAGGTT18310.12453164606895557No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17870.12153907784010028No Hit
CTTTTGGCCAGGGGACCCAACTGGAGATGAAACGAACTGTGGCTGCACCA17810.12113100035434728No Hit
ATAGATGAGGAGCCTGGGAGCCTGGCCACGTTTGTGTTGGTACCAGGTTAAGAAATTGGTATTGACACTCTGG17470.11881856126841366No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17390.11827445795407632No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17020.11575798012526618No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16950.11528188972522102No Hit
CTCCCAGGCTCCTCATCTATAGTGCATCCATCAGGGCCACTGGCGTCCCA16920.11507785098234452No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16640.11317348938216387No Hit
GGCCAAAAGTGAACGAGTCATGGTACTGCTGACAGTAATACACTGCAAAA16520.1123573344106579No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG15750.10712034001016113No Hit
CAGCAGTACCATGACTCGTTCACTTTTGGCCAGGGGACCCAACTGGAGATGAAACGAACTGTGGCTGCACCAT15650.10644021086723948No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15630.10630418503865513No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15560.10582809463861No Hit
GTACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGA15560.10582809463861No Hit
TCCATACACTACGCAGAGAGATTCCAGGGCAGAGTCACCATGACCGGGGA15470.10521597840998048No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15390.10467187509564317No Hit
GTCTGGGACGCCAGTGGCCCTGATGGATGCACTATAGATGAGGAGCCTGGGAGCCTGGCCACGTTTGTGTTGGTA15190.10331161680979983No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14960.10174731978108004No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC14910.10140725520961921No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA53150.040.4642451
GGTATCA21050.038.4562641
TATCAAC61100.035.3676762
ATCAACG64650.033.6378253
TCAACGC65100.033.352614
CGCTAAT2000.032.67825332
CAACGCA66850.032.530825
AACGCAG67450.032.343156
TCGTATG2200.031.41359540
GCCTAAC450.004755765730.4898071
TCGCTAA2500.030.25696231
AATGGTA3600.030.11685670
AGAGTAC73900.029.55458811
CGTATGC2400.028.8204941
ACGAGAC3050.028.1184722
TATGCCG2600.027.9768943
TATACAC3200.027.8695873
ACGCAGA78400.027.8582137
CTTATAC2750.027.4408261
TGCGTAA500.007972890527.42962610
Produced by FastQC (version 0.11.5)