FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2360689
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39840.16876428873095947No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC36710.1555054477739338No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35290.1494902547518966No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT32030.13568072710975482No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG31540.13360506191200958No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACGGCACTCAGGCTGCTATCCCATGTT30570.12949609203075882No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28400.1203038604407442No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTG26820.11361089919087182No Hit
GTAGTAGACGGCCGCGTCGTCAGATCTCAGGCTCCTCACCTCCATGTAGGCTGTGCTCGTGGATGTGTCAGTGGT25240.10691793794099942No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC24370.10323257320214564No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACGGCACTCAGG24060.10191939726071499No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC14250.049.47672
GTGGTAT16000.044.7413831
GTATCAA101200.041.320141
GGTATCA37500.039.923081
TATCAAC111100.037.580832
ATCAACG121100.034.486913
TCAACGC121250.034.4143184
CAACGCA123750.033.7303925
AACGCAG125500.033.2859886
CGCAGAG136250.030.7353328
AGAGTAC136900.030.48910911
ACGCAGA138650.030.1537957
CAGAGTA139500.029.89624810
GCAGAGT141450.029.6297029
AGTACGG96850.026.08732613
GAGTACG98200.025.83192312
GAGTACT64550.025.31246812
AGTACTT64600.024.81622713
GTACTTT66150.024.23782314
TACGGGG40700.023.28353315
Produced by FastQC (version 0.11.5)