FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1114606
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32550.29203144429511413No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21970.1971100101739987No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATG20870.18724105199505475No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20180.1810505236828081No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA19390.1739628173542938No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18280.1640041413737231No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18150.16283780995257519No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17160.1539557475915256No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17030.1527894161703777No Hit
GGTGTATACTACGGAGCCTCTGTGAAGGGCCGTTTCACCAGCTCCAGGGACAACGCCAAGAACTCAGTGTATCTG16840.15108477793946917No Hit
GTAATAGACCGCCGTGTCATCGGCTCTCAGTCTGTTCATTTGCAGATACA16350.14668860565975778No Hit
GTGCAGCCACAGTTCGTTTGATCTCTACCTTGGTCCCTCCGCCGAAAGTGAGCTCCGGAGGCCAGTAGCTACG16070.1441765072142084No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15550.13951118152961675No Hit
GCCTAGAGCCTGAAGATGTTGCAGTTTATTACTGTCAGCAGCGTAGCTAC15480.13888315691822942No Hit
GTCTATTACTGTGTGAGAGATGGCGCCTTCGGATATAGTGGCTACGATCGTGAAGAGTACTGGGGCCAGGGAACC14890.13358980662225037No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT14750.13233375739947567No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14500.13009081235880662No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGGGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAG14490.13000109455717984No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14380.12901419873928546No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGCTCCGGAGGCCAGTAGCTACGCTG14210.12748899611163048No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT13600.12201621021239793No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13480.12093959659287676No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13160.11806862694082035No Hit
AGCCTGGAGAGTCCCTGACACTCTCCTGTGTAGCCTCTGGATTCACCTTC12920.11591539970177801No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12180.10927628238139755No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCTACCTTGGTCCCTCCGCCGAAAGTGAGCTCCGGAGG12180.10927628238139755No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12020.10784079755536934No Hit
GTCTTTGTCTCCAGGGGAAAGGGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCTACTTAGCCTGGTA11850.10631559492771438No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11710.1050595457049397No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11580.10389321428379177No Hit
GTTTATTACTGTCAGCAGCGTAGCTACTGGCCTCCGGAGCTCACTTTCGGCGGAGGGACCAAGGTAGAGATCAAA11490.1030857540691509No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATGTTGCAGTTTATTACTGTCAGCAGCGTAGCTACTGG11450.10272688286264384No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11290.10129139803661563No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11150.10003534881384095No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGC306.2601146E-446.07616438
GTATCAA38300.042.6601141
GGTATCA14400.041.1726151
TATCAAC43350.037.6701552
ATCAACG46100.035.475433
TCAACGC46050.035.405784
CAACGCA46950.034.5681345
GTGGTAT7200.034.4700971
AACGCAG47250.034.3486566
TGGTATC7500.032.6140862
CGCAGAG51300.031.8380038
ACGCAGA52400.030.9071667
GCAGAGT53250.030.542969
CAGAGTA54550.029.8150810
GAGTACG39800.028.5102112
AGTACGG39650.028.36044913
GTATAAC500.00776761327.5760751
AGAGTAC58900.027.55473911
TACGGGA15600.026.24138815
ACGGGGT2600.025.13993816
Produced by FastQC (version 0.11.5)