FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1053477
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT64770.6148212063481215No Hit
GATCTATAGGGCGTCGAGCTTAGAGACTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA57900.5496085818674732No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC43960.4172848576665651No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43550.4133929834253619No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG41700.3958320874589573No Hit
AGCTTAGAGACTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC41530.39421838350528776No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41300.3920351369797347No Hit
CTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC40010.3797899716842418No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT38960.36982297667628244No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG37930.36004582919228423No Hit
GTATGAAAGTTACTCGTACAATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT37630.3571981163328673No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT35060.332802709503862No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAATTG33600.3189438402546994No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33250.3156215085853797No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACTATCACTTGCCG32970.3129636432499238No Hit
GTAGGAGACAGAGTCACTATCACTTGCCGGGCCAGTCAGAGGATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAA32770.31106516801031253No Hit
GTCTGCATCTGTAGGAGACAGAGTCACTATCACTTGCCGGGCCAGTCAGA30580.2902768641365687No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG28930.2746144434097755No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGACATGGCAACTTATTACTGCCAACAGTATGAAAGTTA28880.27413982459987263No Hit
CTCTAAGCTCGACGCCCTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTT28050.2662611523554857No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26690.2533515207261288No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25990.24670685738748924No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT25870.24556777224372245No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC25590.24290990690826664No Hit
CTTTCATACTGTTGGCAGTAATAAGTTGCCATGTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC25570.2427200593843055No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT25360.24072666038271362No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25140.2386383376191412No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23550.2235454594642313No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC22500.21357846445627196No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21740.206364258545749No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG21620.2052251734019822No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21550.20456070706811824No Hit
GTATCAACGCAGAGTACGGGAGTCAGACCCAGTCAGGACACAGCATGGAC21020.19952974768314827No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20250.19222061801064474No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20020.19003737148509176No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19990.18975260019915005No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19800.1879490487215193No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19610.18614549724388857No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19610.18614549724388857No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACTATCACTTGCCGGGCCAGTCAGAGGATTAGTAG19470.18481656457616066No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18850.1789312913333656No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18510.17570388342602639No Hit
ACTTATTACTGCCAACAGTATGAAAGTTACTCGTACAATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA18350.17418510323433734No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18220.17295109432859No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAGGGCGTCGAGCTTAGAGACTGGGGTCCCAT18170.17247647551868717No Hit
GCCTGATGACATGGCAACTTATTACTGCCAACAGTATGAAAGTTACTCGTACAATTTTGGCCAGGGGACCAAG17960.1704830765170953No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17590.16697089732381437No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC17310.16431303198835856No Hit
GTCAGAGGATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAGGG17260.16383841317845574No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGACATGG16550.1570988260778356No Hit
CACCATCAGCAGCCTGCAGCCTGATGACATGGCAACTTATTACTGCCAAC16510.15671913102991333No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16400.1556749696481271No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16310.15482065579030202No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16290.15463080826634087No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16080.15263740926474903No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16070.15254248550276844No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15630.14836583997562358No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC15300.14523335583026492No Hit
GCCTGCAGCCTGATGACATGGCAACTTATTACTGCCAACAGTATGAAAGTTACTCGTACAATTTTGGCCAGGGGA15000.14238564297084796No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14500.1376394548718197No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14460.13725975982389743No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC14350.13621559844211123No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC14250.1352663608223056No Hit
CAGTATGAAAGTTACTCGTACAATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT14230.13507651329834444No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14190.13469681825042218No Hit
ATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA13810.1310897152951607No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13800.13099479153318014No Hit
CACTATCACTTGCCGGGCCAGTCAGAGGATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCC13780.130804944009219No Hit
ACCTTGATGGGACCCCAGTCTCTAAGCTCGACGCCCTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTG13590.12900139253158827No Hit
TCTATAGGGCGTCGAGCTTAGAGACTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT13520.1283369261977243No Hit
CCCCTAAGCTCCTGATCTATAGGGCGTCGAGCTTAGAGACTGGGGTCCCA13490.1280521549117826No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC13310.12634352719613243No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13170.12501459452840452No Hit
GTAATAAGTTGCCATGTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG13150.12482474700444338No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT13130.12463489948048224No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12700.12055317771531794No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12580.11941409257155117No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG12570.1193191688095706No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT12510.11874962623768721No Hit
ATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGACA12510.11874962623768721No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12370.11742069356995928No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG12290.11666130347411476No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12240.11618668466421193No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12240.11618668466421193No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12170.11552221833034798No Hit
GCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTAGGAGA12090.11476282823450347No Hit
GGCCAAAATTGTACGAGTAACTTTCATACTGTTGGCAGTAATAAGTTGCC11890.11286435299489214No Hit
GATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAGGGCGTCGAG11750.11153542032716424No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11730.1113455728032031No Hit
CTGTAGGAGACAGAGTCACTATCACTTGCCGGGCCAGTCAGAGGATTAGT11690.11096587775528086No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC11590.1100166401354752No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11520.10935217380161123No Hit
GGATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT11490.10906740251566953No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11480.10897247875368897No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11460.10878263122972784No Hit
GAGGATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCC11460.10878263122972784No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11360.1078333936099222No Hit
GCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAGGGCGTCGAGCTTAGAGACTGGGGTCCCATCAAGGTT11320.10745369856199993No Hit
GAGTCACTATCACTTGCCGGGCCAGTCAGAGGATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAG11300.1072638510380388No Hit
GTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCC11300.1072638510380388No Hit
CTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACTATCACTTGCCGG11250.10678923222813598No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11150.10583999460833032No Hit
ATAGGGCGTCGAGCTTAGAGACTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCA11070.10508060451248578No Hit
GTATCAACGCAGAGTACGGGGAGTCAGACCCAGTCAGGACACAGCATGGA11070.10508060451248578No Hit
CCCTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTAATCCTCTGA10820.10270751046297166No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10620.10080903522336035No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGC10560.10023949265147697No Hit
GGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC10550.1001445688894964No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10550.1001445688894964No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG10540.10004964512751584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3200.052.6051
CGCATCG200.0074605951.57060626
CTACGCT2450.040.7203328
CTTATAC2600.039.6395651
GTATCAA29450.039.3120351
CGAGACT2700.038.18049223
ACTACGC2650.037.6345727
ACGAGAC2750.037.48096522
CACGAGA2750.037.47918321
TATACAC3100.036.5689543
CCCACGA2850.036.15554419
GAGACTA2950.036.1148324
ATCTCCG2950.036.0891210
TCCGAGC3100.035.45070613
TACGCTG2750.035.038829
CGAGCCC3050.034.90752815
TACACAT3200.034.3526575
CCGAGCC3200.034.34287314
TATCAAC34750.033.2158052
TTATACA3200.032.2056162
Produced by FastQC (version 0.11.5)