FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1053477
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT61900.5875780866596992No Hit
GATCTATAGGGCGTCGAGCTTAGAGACTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA49730.47205586832935126No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT39140.3715316043919326No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38170.36232399947981775No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC37870.3594762866204008No Hit
GTATGAAAGTTACTCGTACAATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT37470.3556793361411782No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36580.3472311213249079No Hit
AGCTTAGAGACTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC35580.33773874512685137No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34750.32986007288246444No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAATTG34380.3263478936891835No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT33680.319703230350544No Hit
CTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC32580.30926161653268175No Hit
CTTTCATACTGTTGGCAGTAATAAGTTGCCATGTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC30930.2935991958058885No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29520.2802149453666288No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGACATGGCAACTTATTACTGCCAACAGTATGAAAGTTA29250.2776520037931535No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28110.2668306949273691No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26450.25107335043859524No Hit
CTCTAAGCTCGACGCCCTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTT25530.24234036433638326No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25480.2418657455264804No Hit
GTCTGCATCTGTAGGAGACAGAGTCACTATCACTTGCCGGGCCAGTCAGA25420.241296202954597No Hit
GTAGGAGACAGAGTCACTATCACTTGCCGGGCCAGTCAGAGGATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAA25240.23958757523894683No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24890.23626524356962705No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC24810.23550585347378253No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACTATCACTTGCCG24710.23455661585397689No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22660.21509724464796098No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT22530.21386323574221364No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22260.21130029416873836No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21810.2070287248796129No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG21790.2068388773556518No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21370.20285207935246807No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20900.1983906625393815No Hit
GTATCAACGCAGAGTACGGGAGTCAGACCCAGTCAGGACACAGCATGGAC20890.19829573877740092No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT20530.19487848334610058No Hit
ACTTATTACTGCCAACAGTATGAAAGTTACTCGTACAATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA20170.19146122791480022No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20120.1909866091048974No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19990.18975260019915005No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19810.18804397248349985No Hit
GCCTGATGACATGGCAACTTATTACTGCCAACAGTATGAAAGTTACTCGTACAATTTTGGCCAGGGGACCAAG19060.18092469033495748No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18780.17826682499950164No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC18450.17513434085414298No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18190.1726663230426483No Hit
GTAATAAGTTGCCATGTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG18150.17228662799472605No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18140.1721917042327455No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17930.1701983052311536No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17840.1693439913733285No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17750.16848967751550342No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16760.15909222507942744No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16720.15871253003150518No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16440.15605466469604937No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16080.15263740926474903No Hit
ATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA15610.14817599245166246No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACTATCACTTGCCGGGCCAGTCAGAGGATTAGTAG15440.14656228849799283No Hit
CACCATCAGCAGCCTGCAGCCTGATGACATGGCAACTTATTACTGCCAAC15260.14485366078234266No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA15260.14485366078234266No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15040.14276533801877023No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14910.14153132911302288No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC14560.13820899744370307No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAGGGCGTCGAGCTTAGAGACTGGGGTCCCAT14560.13820899744370307No Hit
GCCTGCAGCCTGATGACATGGCAACTTATTACTGCCAACAGTATGAAAGTTACTCGTACAATTTTGGCCAGGGGA14420.13688006477597517No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGACATGG14070.1335577331066554No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14040.1332729618207137No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13910.13203895291496634No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13870.13165925786704408No Hit
GGCCAAAATTGTACGAGTAACTTTCATACTGTTGGCAGTAATAAGTTGCC13400.1271978410539575No Hit
GGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCAGT13110.12444505195652111No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12930.12273642424087096No Hit
GTCAGAGGATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAGGG12830.12178718662106529No Hit
ATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGACA12830.12178718662106529No Hit
ACCTTGATGGGACCCCAGTCTCTAAGCTCGACGCCCTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTG12820.12169226285908472No Hit
GTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGAC12650.12007855890541512No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC12640.11998363514343455No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12590.11950901633353173No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12550.11912932128560946No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12550.11912932128560946No Hit
CAGTATGAAAGTTACTCGTACAATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT12370.11742069356995928No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12240.11618668466421193No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12170.11552221833034798No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC11890.11286435299489214No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11830.11229481042300876No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11520.10935217380161123No Hit
CACTATCACTTGCCGGGCCAGTCAGAGGATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCC11510.10925725003963066No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11460.10878263122972784No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11430.10849785994378615No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11300.1072638510380388No Hit
TCTATAGGGCGTCGAGCTTAGAGACTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT11280.10707400351407767No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT11170.10602984213229145No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC11110.10546029956040805No Hit
CTCCAGCTTGGTCCCCTGGCCAAAATTGTACGAGTAACTTTCATACTGTT10950.10394151936871901No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC10920.10365674808277732No Hit
GTATCAACGCAGAGTACGGGGAGTCAGACCCAGTCAGGACACAGCATGGA10890.10337197679683563No Hit
GCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAATTGTACGA10830.10280243422495224No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT10720.10175827284316602No Hit
GCTTGGTCCCCTGGCCAAAATTGTACGAGTAACTTTCATACTGTTGGCAGTAATAAGTTGCCATGTCATCAGG10700.10156842531920489No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10650.10109380650930204No Hit
GTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCC10640.10099888274732148No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)