FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1162465
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT63950.5501240897575411No Hit
GGGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAAT57930.4983375843573785No Hit
CTACAAACCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACG55320.47588529547126157No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG47350.40732409147802295No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT42630.3667207184732444No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41830.3598387908453157No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38820.3339455381452345No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38010.3269775864219568No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37860.3256872249917202No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA37180.3198375865079809No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA35440.3048693939172362No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG35200.30280481562885764No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34910.30031011686373354No Hit
GGTCTAGTCAGAGCCTCCTGCATACTAATGGAAGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGT33930.291879755519521No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT33700.28990120132649155No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA32410.2788040930264567No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA31830.2738146954962085No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT31180.2682231292985165No Hit
GGCCAAAAGTGTACGGGGTTTGTAGAGCTTGCATGCAGTAATAAACCCCA29900.2572120450938308No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29790.25626578004499057No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC29320.2522226475635826No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28020.2410395151681986No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG26350.2266734912448977No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGTAGAG25310.21772698532859053No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC25020.2152322865634664No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24490.21067300950996376No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTCGGGTTCTAATCGGGCC24210.20826433484018875No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24120.20749011798204675No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23870.20533951559831906No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA23410.20138240721226017No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCGAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA23310.20052216625876906No Hit
GATTTTACACTGAAAATCAGTAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACC23300.20043614216341998No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23240.1999199975913253No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23220.19974794940062712No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23150.19914578073318337No Hit
TTATTACTGCATGCAAGCTCTACAAACCCCGTACACTTTTGGCCAGGGGA22900.19699517834945568No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG22660.1949306000610771No Hit
CTAATGGAAGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAG22330.19209180491455657No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCAGTCTCCCTGCCCGTCACCC21620.18598409414476996No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21080.18133879299591815No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCAGT20980.18047855204242708No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA20650.17763975689590655No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20150.17333855212845117No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20020.17222023888891277No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20020.17222023888891277No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20000.17204819069821456No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCAGTCTCC19860.17084385336332705No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA19810.17041373288658154No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19470.16748891364471188No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18960.16310168478190742No Hit
GTTTATTACTGCATGCAAGCTCTACAAACCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA18850.16215541973306724No Hit
AGTCAGAGCCTCCTGCATACTAATGGAAGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCA18480.15897252820515026No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18470.15888650410980115No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18330.15768216677491365No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18140.15604770896328063No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC17980.1546713234376949No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG17650.15183252829117436No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT17600.15140240781442882No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTC17340.14916578133535202No Hit
GTCTCCAGTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT16840.14486457656789667No Hit
CTCCACAGCTCCTGATCTATTCGGGTTCTAATCGGGCCTCCGGGGTCCCT16490.14185373323067793No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTCGGGTTCTAATCGGGCCTCCGGGGTCCCTGA16270.13996120313299754No Hit
GTCTCCACAGCTCCTGATCTATTCGGGTTCTAATCGGGCCTCCGGGGTCC16150.13892891398880827No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15720.13522987788879665No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15490.13325132369576717No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC15420.13264915502832342No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT15310.13170288997948323No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15200.13075662493064308No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCTTCCATTAGTA15180.13058457673994486No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14820.12748770930737702No Hit
GTGTACGGGGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTC14710.1265414442585368No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14670.12619734787714038No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14540.125079034637602No Hit
GTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCTTCCATTAGTATGCAGGAGGCTCTGA14440.12421879368411091No Hit
GGTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCAC14350.12344457682596896No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14090.12120795034689218No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14050.12086385396549573No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14010.1205197575840993No Hit
GCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTCGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTT13830.11897132386781539No Hit
CCTGCATACTAATGGAAGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGAT13660.11750891424688055No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13570.11673469738873858No Hit
GGGATATTGTGATGACTCAGTCTCCAGTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGT13560.11664867329338947No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13400.11527228776780375No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13340.11475614319570912No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC13270.11415397452826537No Hit
GCTCCTGATCTATTCGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGT13010.11191734804918858No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13000.11183132395383946No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12990.11174529985849037No Hit
AGTCTCCACAGCTCCTGATCTATTCGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGGAT12970.11157325166779215No Hit
CCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC12690.10916457699801714No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12620.10856240833057339No Hit
GGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGGATC12370.1064118059468457No Hit
GGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCAGTCTCCCTGCCCGTCACCCCTGGAGAGCCGG12240.10529349270730733No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC12210.10503542042126No Hit
GATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAAT12190.10486337223056179No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT12170.10469132403986357No Hit
GAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATG12160.10460529994451445No Hit
GTAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTA12120.10426120356311804No Hit
CTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT12100.10408915537241982No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT12030.10348698670497607No Hit
GGCACAGATTTTACACTGAAAATCAGTAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTA11960.10288481803753231No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11850.10193855298869213No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA11810.10159445660729571No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11780.10133638432124838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4400.053.861741
TGGTATC4400.050.706542
GGTATCA14800.047.8067781
GTATCAA41500.042.7884331
GAACGAA350.001203514640.3471763
TATCAAC47000.037.3917162
ATCAACG49800.035.3430673
TCAACGC50050.034.880314
CGTACTT400.00268016234.2771765
CAACGCA51200.034.2102285
AACGCAG52600.033.819566
CGCAGAG55550.031.9001568
ACGCAGA55700.031.8142497
AGAGTAC57000.031.50959411
ATAAACG1101.1241355E-931.1905883
GCAGAGT57200.030.979969
CAGAGTA57550.030.91066610
GAGTACG40050.027.64307612
AGTACGG39900.027.57637413
TCGATCT500.00798508427.4205639
Produced by FastQC (version 0.11.5)