FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1377078
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16520.11996415598825919No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA56900.039.6226961
GGTATCA24100.035.6513061
GTGGTAT9400.034.367861
TATCAAC66750.033.518322
ATCAACG67550.033.3248673
TCAACGC68300.032.908614
CAACGCA69600.032.3433155
AACGCAG72300.031.0879386
TGGTATC10700.030.8347132
ACGCAGA77650.028.9355037
CGCAGAG78800.028.6004188
TCGTATG1700.028.5466140
AGAGTAC81800.027.677511
CAGAGTA83900.027.02568410
GCAGAGT84250.026.7503019
CGTATGC2200.023.66124341
GAGTACG52000.023.38808612
AGTACGG51900.023.36695513
TATCGCC750.002041208622.90608220
GAGTACT46250.022.58169712
Produced by FastQC (version 0.11.5)