FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1377078
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29430.2137133844270259No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA71400.040.5946241
GGTATCA29200.038.1053921
GTGGTAT10600.037.905681
TATCAAC81200.035.4234662
ATCAACG84650.033.9612123
TCAACGC84600.033.7466284
CAACGCA86050.033.1273165
AACGCAG87850.032.4473766
ACGCAGA92000.030.9837157
TGGTATC12700.030.8108842
CGCAGAG93250.030.5316458
AGAGTAC95250.030.07040811
CAGAGTA96100.029.76878410
GCAGAGT96950.029.507799
GAGTACG61100.025.34425212
GAGTACT52350.025.32657812
AGTACGG61050.025.1441913
CGAATAG700.001189097925.1337358
TATATCG700.001388861724.4729185
AGTACTT53800.024.13805413
Produced by FastQC (version 0.11.5)