FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences937143
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51120.5454877217244327No Hit
GGGTATTAGCGGCTGGTTAACCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGCTGCATC41420.4419816399418232No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34190.36483226145849673No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32220.34381092319955436No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31620.3374084851511455No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC31300.3339938515253275No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31030.33111275440354354No Hit
GTAGGAGACAGAGTCATCATCACTTGTCGGGCGAGTCAGGGTATTAGCGGCTGGTTAACCTGGTATCAGCAAAAA30830.3289786083874072No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28710.30635666061636274No Hit
CTGTTAGCCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAAATCTGTC28380.30283531968973787No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG28190.30080788097440836No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25240.2693292272363983No Hit
GGCTAACAGTTTCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT24610.26260666728556903No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23540.2511889860992399No Hit
GATCTATGCTGCATCCACTTTGCAAAGTGGAGTCTCATCAAGATTCAGCGGCAGTGGATCTGGGACAGATTTCA23270.24830788897745598No Hit
GGCCAAAAGTGTACGGGAAACTGTTAGCCTGTTGACAGTAGTAAGTTGCA21610.23059447704352484No Hit
ATGCTGCATCCACTTTGCAAAGTGGAGTCTCATCAAGATTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCA21240.22664630691367274No Hit
ACTTACTACTGTCAACAGGCTAACAGTTTCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA21190.22611277040963865No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGCCTCCAGATGCGTCAT20880.22280484408462742No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA20750.22141764917413884No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGGAAA20590.2197103323612298No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20170.21522862572734364No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20120.2146950892233096No Hit
CTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGAGTCTCATCAAGATT20030.21373472351604828No Hit
GGTTAACCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAACTCCTGATC19070.2034908226385941No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18920.2018902131264919No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG18510.19751521379341255No Hit
GTGGAGTCTCATCAAGATTCAGCGGCAGTGGATCTGGGACAGATTTCACT18310.19538106777727626No Hit
AGTCAGGGTATTAGCGGCTGGTTAACCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAACTCCTGATCTATG17950.19153960494823094No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17710.18897862972886742No Hit
GTAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAAATCTGTCCCAGATCCACTGCCG17170.18321643548529948No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGC16560.17670729013608383No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16230.17318594920945896No Hit
CATCAAGATTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTG16170.17254570540461808No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15740.16795729146992508No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14850.15846034169811865No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14710.15696643948682323No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14390.1535518058610052No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14360.15323168395858477No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14330.15291156205616432No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14280.15237802555213023No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13990.14928351382873264No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13970.149070099227119No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13440.1434146122843579No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13320.14213412467467612No Hit
ATCTGGGACAGATTTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTACTGTCAACAGG13310.14202741737386929No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13310.14202741737386929No Hit
ATCTTGATGAGACTCCACTTTGCAAAGTGGATGCAGCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTTTG13100.1397865640569262No Hit
GTCTCCATCTTCTGTGTCTGCATCTGTAGGAGACAGAGTCATCATCACTTGTCGGGCGAGTCAGGGTATTAGCGG12960.1382926618456308No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12670.1351981501222332No Hit
ATCCAGATGACCCAGTCTCCATCTTCTGTGTCTGCATCTGTAGGAGACAGAGTCATCATCACTTGTCGGGCGAGT12500.13338412600851737No Hit
ACTGTCAACAGGCTAACAGTTTCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG12480.13317071140690376No Hit
GTATTAGCGGCTGGTTAACCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGCTGCATCCA12180.12996949238269934No Hit
GCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGT12110.12922254127705163No Hit
ACCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGAGT12080.12890241937463118No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12060.12868900477301756No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11840.12634144415526766No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11830.12623473685446085No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11790.1258079076512336No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11730.12516766384639272No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11560.12335363973267686No Hit
CCCCTAAACTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGAGTCTCA11540.12314022513106324No Hit
GATTTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTACTGTCAACAGGCTAACAGTTTC11530.12303351783025643No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11470.12239327402541553No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGCCTCCAGATGCGTCATCC11410.12175303022057467No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11170.11919205500121113No Hit
CTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTACTGTCA11010.1174847381883021No Hit
CTCCAGATGCGTCATCCAGATGACCCAGTCTCCATCTTCTGTGTCTGCATCTGTAGGAGACAGAGTCATCATCA10850.11577742137539308No Hit
GTCTCTGGTGGCTCCATCAATAGTGGTGGTTACTACTGGAGCTGGATCCG10720.11439022646490452No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10610.11321644615602956No Hit
CTAATACCCTGACTCGCCCGACAAGTGATGATGACTCTGTCTCCTACAGA10600.11310973885522273No Hit
CTGTAGGAGACAGAGTCATCATCACTTGTCGGGCGAGTCAGGGTATTAGC10600.11310973885522273No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10430.1112957147415069No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10290.10980181253021151No Hit
GTTGACAGTAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAAATCTGTCCCAGATCCA10200.10884144682295019No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCC10170.10852132492052975No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10110.10788108111568885No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10090.10766766651407524No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10080.10756095921326841No Hit
ATGCAGCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGTTAACCAGCCGCTAATACC10080.10756095921326841No Hit
CACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTACTGTCAAC9970.10638717890439346No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9930.10596034970116619No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC9830.10489327669309806No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGTTAACC9710.10361278908341628No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT9660.10307925257938223No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC9660.10307925257938223No Hit
CATCATCACTTGTCGGGCGAGTCAGGGTATTAGCGGCTGGTTAACCTGGTATCAGCAAAAACCAGGGAAAGCCC9410.10041157005921189No Hit
GCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGG9390.10019815545759825No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)