FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences937143
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47220.5038718744097752No Hit
CTGTTAGCCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAAATCTGTC32750.3494664101423155No Hit
GGGTATTAGCGGCTGGTTAACCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGCTGCATC32360.3453048254108498No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30810.3287651937857936No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29270.3123322694615443No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG28410.3031554415921583No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27020.28832312678001115No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25750.2747712995775458No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC25260.2695426418380119No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24570.26217983808234174No Hit
GTAGGAGACAGAGTCATCATCACTTGTCGGGCGAGTCAGGGTATTAGCGGCTGGTTAACCTGGTATCAGCAAAAA23980.2558841073347397No Hit
GATCTATGCTGCATCCACTTTGCAAAGTGGAGTCTCATCAAGATTCAGCGGCAGTGGATCTGGGACAGATTTCA23020.2456402064572856No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA22530.24041154871775172No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21720.2317682573523998No Hit
CTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGAGTCTCATCAAGATT21430.2286737456290022No Hit
ACTTACTACTGTCAACAGGCTAACAGTTTCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA21270.22696642881609316No Hit
GGCCAAAAGTGTACGGGAAACTGTTAGCCTGTTGACAGTAGTAAGTTGCA21180.2260060631088318No Hit
GGCTAACAGTTTCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT20910.2231249659870479No Hit
GTAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAAATCTGTCCCAGATCCACTGCCG20720.22109752727171839No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20080.2142682600200823No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19950.21288106510959373No Hit
ATGCTGCATCCACTTTGCAAAGTGGAGTCTCATCAAGATTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCA19890.21224082130475286No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19870.21202740670313922No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGGAAA18550.19794204299663978No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGCCTCCAGATGCGTCAT17930.19132619034661733No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17050.1819359478756177No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16940.18076216756674277No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16530.17638716823366338No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16230.17318594920945896No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15630.16678351116105014No Hit
GTGGAGTCTCATCAAGATTCAGCGGCAGTGGATCTGGGACAGATTTCACT15500.16539631625056156No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15440.16475607244572066No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15410.16443595054330024No Hit
GGTTAACCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAACTCCTGATC15290.16315546293361846No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15120.16134143881990262No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14170.1512042452432553No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14140.15088412334083484No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14140.15088412334083484No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14030.1497103430319599No Hit
AGTCAGGGTATTAGCGGCTGGTTAACCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAACTCCTGATCTATG14030.1497103430319599No Hit
CATCAAGATTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTG13990.14928351382873264No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGC13970.149070099227119No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13850.14778961161743726No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13760.14682924591017593No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13350.14245424657709657No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13170.1405335151625739No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12840.13701217423594905No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12550.13391766251255144No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12490.13327741870771057No Hit
ATCTGGGACAGATTTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTACTGTCAACAGG11970.12772863906575624No Hit
ACTGTCAACAGGCTAACAGTTTCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG11740.12527437114719953No Hit
ATCTTGATGAGACTCCACTTTGCAAAGTGGATGCAGCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTTTG11680.12463412734235862No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11590.12367376163509732No Hit
GTTGACAGTAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAAATCTGTCCCAGATCCA11540.12314022513106324No Hit
GATTTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTACTGTCAACAGGCTAACAGTTTC11320.12079266451331333No Hit
CTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTACTGTCA11190.11940546960282476No Hit
CCCCTAAACTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGAGTCTCA11170.11919205500121113No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10920.11652437248104078No Hit
GTCTCCATCTTCTGTGTCTGCATCTGTAGGAGACAGAGTCATCATCACTTGTCGGGCGAGTCAGGGTATTAGCGG10710.1142835191640977No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10630.11342986075764318No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGCCTCCAGATGCGTCATCC10460.11161583664392734No Hit
ATCCAGATGACCCAGTCTCCATCTTCTGTGTCTGCATCTGTAGGAGACAGAGTCATCATCACTTGTCGGGCGAGT10370.110655470936666No Hit
CTAATACCCTGACTCGCCCGACAAGTGATGATGACTCTGTCTCCTACAGA10340.11033534903424558No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10130.10809449571730248No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10060.1073475446116548No Hit
GTCCCAGATCCACTGCCGCTGAATCTTGATGAGACTCCACTTTGCAAAGT10000.1067073008068139No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9960.10628047160358665No Hit
ACCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGAGT9940.10606705700197301No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9900.10564022779874577No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9870.10532010589632533No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC9870.10532010589632533No Hit
GTATTAGCGGCTGGTTAACCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGCTGCATCCA9560.10201217957131409No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCC9400.10030486275840506No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)