FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679992
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGCTTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32200.4735349827645031No Hit
ACCTTGAGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC27880.41000482358615986No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22970.33779809174225583No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGCTTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22650.3330921540253415No Hit
GAGTTACACTACCTTGAGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT22640.3329450934716879No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20820.3061800727067377No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20390.29985646889963413No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19190.2822092024612054No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18600.27353262979564463No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAACGTC17700.2602971799668231No Hit
CCTCAAGGTAGTGTAACTCTGTTGACAGTAATAAGTTGCAAAGTCTTCAG17040.25059118342568737No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16980.24970882010376594No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15890.23367921975552655No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG15780.2320615536653372No Hit
GTGTAACTCTGTTGACAGTAATAAGTTGCAAAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAGT15750.23162037200437652No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15480.22764973705573008No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14940.21970846715843717No Hit
ATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGTACCTTTTTAAATTGGTATCAGCAGAAA14910.21926728549747643No Hit
GTCTCCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGTAC13150.1933846280544477No Hit
ACACTACCTTGAGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGA13010.1913257803032977No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12990.19103165919599055No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12970.1907375380886834No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAAGCG12470.18338451040600479No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGCTTGGAGGGCGTTATCCACCTTCCACTGTA12350.1816197837621619No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12320.18117860210120118No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC12170.1789726937963976No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12070.17750208825986188No Hit
ATCTGAGACAGACTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGACT11910.17514911940140473No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11900.17500205884775114No Hit
AGGTAGTGTAACTCTGTTGACAGTAATAAGTTGCAAAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAGT11870.17456087718679042No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11850.1742667560794833No Hit
GTATTAGTACCTTTTTAAATTGGTATCAGCAGAAACCAGGGAGAGCCCCTAAGCTCCTGATCTTTGCTGCATCCA11760.17294321109660113No Hit
ACTTATTACTGTCAACAGAGTTACACTACCTTGAGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAA11750.17279615054294756No Hit
CCTGAAGACTTTGCAACTTATTACTGTCAACAGAGTTACACTACCTTGAG11740.17264908998929399No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG11620.17088436334545112No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC11480.16882551559430112No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGCTTGGAGGGCGTTATCCA11330.1666196072894975No Hit
GTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAGTCTGTCTCAGATCCACTGCCACTGAACCTTGATGGGAC11090.1630901540018118No Hit
GACTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGACTTTGCAACTTATTACTGTCAACAGAGTTACACTAC11070.16279603289450464No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10960.16117836680431535No Hit
GTCTGCAACCTGAAGACTTTGCAACTTATTACTGTCAACAGAGTTACACTACCTTGAGGACGTTCGGCCAGGGGA10580.1555900657654796No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10570.15544300521182602No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10400.1529429757997153No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT10120.14882528029741526No Hit
GTAATAAGTTGCAAAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAGTCTGTCTCAGATCCACTGCCA9800.14411934258050094No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9750.1433840398122331No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9650.14191343427569736No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAAGCGGGTAACTCCCAGGAGAG9480.13941340486358664No Hit
CACCATCAGCAGTCTGCAACCTGAAGACTTTGCAACTTATTACTGTCAACAGAGTTACACTACCTTGAGGACGTT9320.13706043600512946No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC9220.13558983046859374No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9200.1352957093612866No Hit
GAGTATTAGTACCTTTTTAAATTGGTATCAGCAGAAACCAGGGAGAGCCCCTAAGCTCCTGATCTTTGCTGCATC9150.13456040659301874No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGAGACAGACTTCACT9130.1342662854857116No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8850.13014858998341156No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGCTTGGAGGGC8850.13014858998341156No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8850.13014858998341156No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8820.12970740832245084No Hit
GTGGAAGGTGGATAACGCCCTCCAAGCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8710.12808974223226155No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC8330.12250144119342582No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT8120.1194131695667008No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8060.11853080624477934No Hit
GTTCAGTGGCAGTGGATCTGAGACAGACTTCACTCTCACCATCAGCAGTC8020.11794256403016506No Hit
GTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA8000.11764844292285791No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7940.11676607960093648No Hit
CTGCAAAGTGGATGCAGCAAAGATCAGGAGCTTAGGGGCTCTCCCTGGTT7710.11338368686690432No Hit
CTATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGT7530.11073659690114002No Hit
GAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGAGACAGACTTCACTCTCACCATCAGCAGTCTGCAAC7520.11058953634748643No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGCTTGGAGGGCGTTATCCACCTTCCA7490.11014835468652572No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC7460.109707173025565No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGCTTGGAGGGCGTTATCCACC7320.10764832527441498No Hit
GAGTGAAGTCTGTCTCAGATCCACTGCCACTGAACCTTGATGGGACCCCA7070.10397181143307568No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC7050.10367769032576854No Hit
CTCCTGATCTTTGCTGCATCCACTTTGCAGAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGAGACAGA7030.1033835692184614No Hit
GTATCAACGCAGAGTACGGGAGTCAGTCTCAGTCAGGACACAGCATGGAC6970.10250120589653997No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA6930.10191296368192568No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC6930.10191296368192568No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT6920.10176590312827209No Hit
CCATCAGCAGTCTGCAACCTGAAGACTTTGCAACTTATTACTGTCAACAG6880.1011776609136578No Hit
GCAGAAACCAGGGAGAGCCCCTAAGCTCCTGATCTTTGCTGCATCCACTTTGCAGAGTGGGGTCCCATCAAGGTT6880.1011776609136578No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC6820.10029529759173637No Hit
GTCTCAGATCCACTGCCACTGAACCTTGATGGGACCCCACTCTGCAAAGT6810.10014823703808279No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3100.054.0507131
CGTTCTT200.007642558351.25524523
GTATCAA28300.042.6535641
TATCAAC31550.038.0065882
ATCAACG33700.035.667453
TCAACGC34050.035.3776784
CAACGCA34650.034.7599645
AACGCAG35250.034.1683046
TTCGGTG400.002727658734.15255720
CGGGTAT501.9806149E-434.13496817
ACGGGAT807.78673E-834.13245816
CGCAGAG37500.032.027228
AGAGTAC38400.030.92116511
ACGCAGA39350.030.6949127
TCTGTCG450.004878694730.32880810
ACGGGTA1950.029.75650216
AGTACGG23750.029.16354213
GTAAGCC500.006142792828.93210866
GCGGCGT755.3844525E-428.78090169
GAGTACG24100.028.74000512
Produced by FastQC (version 0.11.5)