FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences587134
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32600.5552395194282737No Hit
CTACAAACCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACG25460.4336318455412223No Hit
GGGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAAT25150.42835196054052393No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22520.38355809747008346No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG19590.333654668269935No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18750.3193478831067525No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18740.3191775642357622No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18240.3106616206862488No Hit
GGTCTAGTCAGAGCCTCCTGCATACTAATGGAAGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGT17630.30027216955584246No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG17160.29226718261929985No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA17140.2919265448773193No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT16780.28579506552166967No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16470.28051518052097135No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA15980.27216955584244823No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA15420.26263169906699324No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15110.2573518140662949No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14870.2532641611625285No Hit
GGCCAAAAGTGTACGGGGTTTGTAGAGCTTGCATGCAGTAATAAACCCCA14340.24423726100004428No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14040.23912769487033622No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA13140.22379899648121213No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTCGGGTTCTAATCGGGCC12990.2212442134163581No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGTAGAG12700.21630496615764033No Hit
TTATTACTGCATGCAAGCTCTACAAACCCCGTACACTTTTGGCCAGGGGA12100.20608583389822424No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG11990.20421232631733133No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11970.20387168857535076No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11930.20319041309138972No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11740.19995435454257462No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC11690.19910276018762327No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11300.19246032421900283No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11230.19126809212207094No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11150.1899055411541488No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10770.18343342405651863No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10730.18275214857255753No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10540.17951609002374247No Hit
CTAATGGAAGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAG10290.17525811824898574No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG10220.17406588615205387No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9890.16844536340937505No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCAGTCTCCCTGCCCGTCACCC9680.16486866711857942No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9660.16452802937659886No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCGAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA9650.1643577105056086No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9650.1643577105056086No Hit
GATTTTACACTGAAAATCAGTAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACC9530.16231388405372538No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9480.16146228969877405No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCAGT9380.15975910098887136No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA9280.1580559122789687No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC9040.15396825937520225No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8860.15090251969737742No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT8760.14919933098747473No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8760.14919933098747473No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA8620.146814866793611No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8410.14323817050281537No Hit
AGTCAGAGCCTCCTGCATACTAATGGAAGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCA8370.1425568950188543No Hit
GTTTATTACTGCATGCAAGCTCTACAAACCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA8260.14068338743796135No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTC8240.14034274969598082No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8240.14034274969598082No Hit
CTCCACAGCTCCTGATCTATTCGGGTTCTAATCGGGCCTCCGGGGTCCCT8220.14000211195400028No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8180.1393208364700392No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCAGTCTCC7990.13608477792122411No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT7790.13267840050141874No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTCGGGTTCTAATCGGGCCTCCGGGGTCCCTGA7770.13233776275943823No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA7760.13216744388844795No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT7630.12995329856557447No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC7470.12722819662973017No Hit
GTCTCCACAGCTCCTGATCTATTCGGGTTCTAATCGGGCCTCCGGGGTCC7450.12688755888774966No Hit
GTCTCCAGTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT7260.12365150033893456No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA7250.12348118146794428No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7000.11922320969318759No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA6920.11786065872526545No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC6810.11598715114437248No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT6740.11479491904744062No Hit
GCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTCGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTT6620.1127510925955574No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6510.11087758501466445No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC6470.11019630953070338No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC6460.11002599065971311No Hit
CCTGCATACTAATGGAAGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGAT6410.10917439630476178No Hit
GTATCAACGCAGAGTACGGGTGATCAGGACTCCTCAGTTCACCTTCTCAC6400.10900407743377151No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC6390.10883375856278123No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG6380.10866343969179097No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC6350.10815248307882017No Hit
GGTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCAC6270.10678993211089803No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCTTCCATTAGTA6230.10610865662693694No Hit
GTGTACGGGGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTC6190.10542738114297588No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT6170.10508674340099534No Hit
AGTCTCCACAGCTCCTGATCTATTCGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGGAT6090.1037241924330732No Hit
GCTCCTGATCTATTCGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGT6080.10355387356208294No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT6030.1027022792071316No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC5960.10151004711019972No Hit
GTAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTA5920.10082877162623866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2600.058.2812771
TGGTATC2650.057.1621172
CGTTTTA200.007466627351.55719814
GGTATCA7550.042.4214671
GTATCAA20150.041.8736421
GTGTAAG350.001359061339.3587841
TATCAAC23350.035.8278582
GTGCACT400.002638829434.383184
ATCAACG24300.034.2621233
TCAACGC24450.034.0316124
CAACGCA25350.033.0777475
AACGCAG25950.032.3129466
CGCAGAG27200.030.8279748
GTATAGA450.004661077630.6123871
AGAGTAC27500.030.49166911
CAGAGTA27900.030.30086310
GCAGAGT27900.030.1776899
AGCATGG900.005124061430.02053570
TGTTAGA5000.029.7203370
ACGCAGA28550.029.3702587
Produced by FastQC (version 0.11.5)