FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3876
Sequences flagged as poor quality0
Sequence length21-76
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT40410.42311661506708No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT1483.8183694530443755TruSeq Adapter, Index 1 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATGCCGT1002.5799793601651184No Hit
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCT741.9091847265221877RNA PCR Primer, Index 1 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGG601.5479876160990713No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATGCCGG320.8255933952528379No Hit
TATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCT310.7997936016511867RNA PCR Primer, Index 1 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTTTGCCGT300.7739938080495357No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATGCCGTCTT280.7223942208462332No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGGCGT270.696594427244582No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG250.6449948400412796No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATGTCGTATGCCGT230.5933952528379773No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC230.5933952528379773No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATTCCGT230.5933952528379773No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGGATGCCGT220.5675954592363261No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATATCGTATGCCGT200.5159958720330238No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGAGCTCGTATGCCGT200.5159958720330238No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCGT180.46439628482972134No Hit
GTGTATAAGAGACAGATACACATCTCCGAGCCCACGAGACATCTCAGGAT160.41279669762641896No Hit
GTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACATCTCAGGA150.38699690402476783No Hit
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTG150.38699690402476783RNA PCR Primer, Index 40 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGGCTT150.38699690402476783No Hit
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTGT140.3611971104231166RNA PCR Primer, Index 1 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATGGCGT130.3353973168214654No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG120.30959752321981426No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATGGCGTATGCCGT110.2837977296181631No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTTTGCCGT110.2837977296181631No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTGTT110.2837977296181631No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTTTGCCGTCTT100.2579979360165119No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGGATGCCGT100.2579979360165119No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTTTGCCGG90.23219814241486067No Hit
ACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATGCCGTCTTCT90.23219814241486067No Hit
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTGTTCT90.23219814241486067No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATGGCGG90.23219814241486067No Hit
TTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC90.23219814241486067RNA PCR Primer, Index 40 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACATCTCAGGATGTCGTATGCCGTCTT90.23219814241486067No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTGGTATGCCGT80.20639834881320948No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGAGCGCGTATGCCGT80.20639834881320948No Hit
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCGTCT80.20639834881320948No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATGCCGGCTT70.1805985552115583No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGAGCTCGTATGCCGG70.1805985552115583No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGGATTCCGT70.1805985552115583No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTG60.15479876160990713TruSeq Adapter, Index 1 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATGTCGTATGCCGG60.15479876160990713No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGGATGGCGT60.15479876160990713No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCGGGTATGCCGT60.15479876160990713No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGGCGG60.15479876160990713No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATATCGTATGCCGTCTT60.15479876160990713No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGGCGT60.15479876160990713No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCCT60.15479876160990713No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGGATGGCGG60.15479876160990713No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGCTCTCGTATGCCGT60.15479876160990713No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTGGT60.15479876160990713No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGAT50.12899896800825594No Hit
CAGCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGC50.12899896800825594No Hit
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGGCTTCT50.12899896800825594No Hit
CTTATACACATCTCCGAGCCCACG50.12899896800825594No Hit
GTATAAGAGACAGATACACATCTCCGAGCCCACGAGACATCTCAGGATCT50.12899896800825594No Hit
GTATAAGAGACAGATACACATCTCCGAGCCCACGAGACATCTCAGGATCG50.12899896800825594No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATAGCGTATGCCGT50.12899896800825594No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGAGCTCGTATGCCGTCTT50.12899896800825594No Hit
GCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG50.12899896800825594No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGGATGCCGG50.12899896800825594No Hit
CTTATACACATCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGA50.12899896800825594No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTTTGCCGG50.12899896800825594No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATGCCG50.12899896800825594No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATTCCGT50.12899896800825594No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTTTGGCGG50.12899896800825594No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATGCCGTGTT50.12899896800825594No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGGCGTCTT50.12899896800825594No Hit
TTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGGC50.12899896800825594No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATGTCGTATGCC40.10319917440660474No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTGGTATGCCGG40.10319917440660474No Hit
ACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATGCCGGCTTCT40.10319917440660474No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTAGTATGCCGT40.10319917440660474No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGGATGGCGG40.10319917440660474No Hit
CCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG40.10319917440660474No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATGGCGTATGCCGG40.10319917440660474No Hit
GAGACAGCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT40.10319917440660474No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATGTCGTATGGCGT40.10319917440660474No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTAGTATGGCGT40.10319917440660474No Hit
GCCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC40.10319917440660474No Hit
GCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATGCCG40.10319917440660474No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCGGGTATGCCGTCTT40.10319917440660474No Hit
GAGACAGCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTC40.10319917440660474No Hit
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTAT40.10319917440660474RNA PCR Primer, Index 1 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTTTGCCGGCTT40.10319917440660474No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)