FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1896081
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38020.20051885969006597No Hit
GTAATAGACCGCCGTGTCATCGGCTCTCAGTCTGTTCATTTGCAGATACA33150.174834302964905No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA33120.17467608187624897No Hit
GGTGTATACTACGGAGCCTCTGTGAAGGGCCGTTTCACCAGCTCCAGGGACAACGCCAAGAACTCAGTGTATCTG28360.1495716691428267No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATG26690.14076402854097478No Hit
GTCTATTACTGTGTGAGAGATGGCGCCTTCGGATATAGTGGCTACGATCGTGAAGAGTACTGGGGCCAGGGAACC26540.13997292309769468No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26100.13765234713073968No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25330.13359133918856841No Hit
AGCCTGGAGAGTCCCTGACACTCTCCTGTGTAGCCTCTGGATTCACCTTC25110.13243105120509094No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24720.13037417705256263No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGGGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAG24640.12995225414947992No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23870.12589124620730865No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23170.12219942080533479No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT22710.11977336411260911No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT21630.1140774049209923No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20670.10901433008399958No Hit
GCCTAGAGCCTGAAGATGTTGCAGTTTATTACTGTCAGCAGCGTAGCTAC20540.10832870536649014No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC20400.10759034028609538No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20150.10627183121396185No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGTTTCCATTCGGTGAT19080.10062861238523038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26500.042.6448331
ACGTTAG350.001256218139.99839461
GTATCAA64850.039.1026571
GTGGTAT11200.034.7615281
TATCAAC73100.034.6895682
ATCAACG80350.031.8167973
TCAACGC80900.031.600494
TGGTATC12250.031.5007132
CAACGCA82300.031.0210745
AACGCAG84150.030.3800336
CGTATGC1850.028.06961441
CGCAGAG91400.028.0374938
ACGCAGA93000.027.5188187
GCAGAGT95600.026.877789
CAGAGTA96800.026.50900310
ACGAGAC3800.026.29030222
CTTATAC4900.025.3130721
CGTAAAT700.001277411224.8273650
AACGCTA700.001345426824.60733623
AGAGTAC104500.024.52274511
Produced by FastQC (version 0.11.5)