FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1896081
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38650.20384150255184247No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32580.17182810228044057No Hit
GTAATAGACCGCCGTGTCATCGGCTCTCAGTCTGTTCATTTGCAGATACA29980.15811560793025192No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26130.1378105682193957No Hit
GGTGTATACTACGGAGCCTCTGTGAAGGGCCGTTTCACCAGCTCCAGGGACAACGCCAAGAACTCAGTGTATCTG25220.13301119519682966No Hit
GTCTATTACTGTGTGAGAGATGGCGCCTTCGGATATAGTGGCTACGATCGTGAAGAGTACTGGGGCCAGGGAACC24130.12726249564232753No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATG23960.12636590947327672No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23880.125943986570194No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA22690.11966788338683844No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22160.11687264415391536No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21850.11523769290446981No Hit
AGCCTGGAGAGTCCCTGACACTCTCCTGTGTAGCCTCTGGATTCACCTTC21390.11281163621174413No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT20380.10748485956032468No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19680.10379303415835082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27200.040.78891
GTATCAA65800.039.377391
GTGGTAT12050.037.457541
TGGTATC12750.036.193582
TATCAAC72850.035.3597342
ATCAACG78150.032.889823
TCAACGC78250.032.692574
CAACGCA79450.032.1894455
AACGCAG81550.031.4861556
CGCAGAG88450.028.9133268
ACGCAGA90650.028.2495427
TTCGGGG13550.028.05228670
CGTCTAA500.007431339527.82827450
GCAGAGT93000.027.6835529
CAGAGTA95550.027.08864210
AGAGTAC102900.025.15241411
AGTACGG65100.024.44749813
GAGTACG66400.024.01935812
AGTACTT44250.022.2170913
TCGGATA11200.022.1322329
Produced by FastQC (version 0.11.5)