FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1113481
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22030.19784800997951468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT7700.041.5257151
GGTATCA19550.040.9764251
GTATCAA50500.039.9642451
TGGTATC8500.037.197862
TATCAAC57350.035.1765942
TCAACGC59600.033.3969044
ATCAACG60250.033.2328573
CAACGCA60800.032.847695
AACGCAG62500.032.3385626
ACGCAGA66300.030.4850717
CGCAGAG67500.029.8922798
AGAGTAC68250.029.76490611
GCAGAGT69700.029.1456939
CAGAGTA71000.028.80536510
GAGTACG44200.026.78442412
AGTACGG45100.026.1750113
ACGGGTA1759.094947E-1225.50153716
TACGGGG19700.024.04555915
CGGGATA1151.7718739E-623.8821117
GTACGGG50150.023.54347214
Produced by FastQC (version 0.11.5)