FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences593437
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8660.14592955949831238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3550.043.5785641
GTATCAA26550.042.6010321
GGTATCA9900.039.2402731
TGGTATC4100.036.0556562
TATCAAC31800.035.892172
TCAACGC33300.034.3786474
ATCAACG33450.034.2244833
CAACGCA33950.033.720445
AACGCAG34800.032.896816
ACGCAGA37500.030.5153757
CGCAGAG37750.030.4017548
AGAGTAC38600.029.82130411
CAGAGTA39800.028.92216910
GCAGAGT40250.028.6841879
GAGTACG24850.026.1339212
CTTATAC1608.185452E-1125.7839831
AGTACGG24950.025.75373513
GAGTACT20650.024.29416312
AGTACTT20800.023.95376813
GTACTTT21500.023.17387814
Produced by FastQC (version 0.11.5)