FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences593437
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18490.31157477541845213No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6140.10346506874360717No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27500.044.1501851
GTGGTAT3550.041.6610871
GGTATCA9650.037.7806171
TATCAAC32800.037.095952
ATCAACG34750.035.19433
TCAACGC34800.035.0993164
CGCTTAC400.002536699634.6610641
ATAAACG400.00264986334.3539923
TTCGCAA501.914855E-434.3308225
CAACGCA35700.034.211585
AACGCAG36550.033.5098236
TGGTATC4350.033.186172
ACGCAGA39050.031.4523627
CGCAGAG39850.030.907048
AGAGTAC40800.029.93513111
CAGAGTA41700.029.6181410
GCAGAGT42150.029.1391419
AGTACGG26250.025.87778913
GAGTACG26350.025.64938412
CCTATAC951.0847476E-525.3134673
Produced by FastQC (version 0.11.5)