FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1200888
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATAGTAAATTACCGCTCACTTTCGGCGGAGGGA37440.3117692907248636No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34190.2847059842383303No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26810.22325146058583314No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA25290.2105941603213622No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC24830.2067636615571144No Hit
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGTTCAGCTTCAGGGAGA23720.19752050149555994No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23420.19502235012757227No Hit
GTGTATTACTGTGCGAGACTTGGCGGTTATTACGATGTTTTGACTGCTTA21610.17995017020737986No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21130.17595312801859955No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20790.17312188980154686No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19990.16646015282024634No Hit
GTCAGGACCCAGAGGGAACCATGGATACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATTA19850.16529434884851876No Hit
GTCTGGGACAGACTTCACTCTCACCATCAACAGACTGGAGCCTGAAGATT18820.15671736248509435No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18680.15555155851336677No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17590.14647494187634483No Hit
GTGTATTACTGTCAGCAGTATAGTAAATTACCGCTCACTTTCGGCGGAGGGACCAAGGTTCAGATCAAACGAA17180.14306080167342833No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16630.13848085749878422No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16600.13823104236198547No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA16560.13789795551292044No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16470.13714851010252413No Hit
GTCTTTGTATCCAGGGGAAAGAGCCACCCTCTCCTGCGGGGCCAGTCAGA16270.135483075857199No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15980.13306819620147758No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15740.13106967510708742No Hit
GTCTCCAGCCACCCTGTCTTTGTATCCAGGGGAAAGAGCCACCCTCTCCTGCGGGGCCAGTCAGACTGTGACCAG14530.1209937979228704No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14300.11907854854074651No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGA14080.11724657087088888No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13990.11649712546049258No Hit
GTATAGTAAATTACCGCTCACTTTCGGCGGAGGGACCAAGGTTCAGATCAAACGAACTGTGGCTGCACCAT13980.11641385374822633No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13610.11333280039437484No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA13580.11308298525757605No Hit
GTGCAGCCACAGTTCGTTTGATCTGAACCTTGGTCCCTCCGCCGAAAGTG13470.11216699642264724No Hit
AACCTGGCCTGGCGCCCAGGCTCCTCATCTATGAGACATCCACCAGGGCC13310.11083464902638715No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC13050.10866958450746447No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12930.1076703239602694No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12810.10667106341307432No Hit
CCTATTACACCCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGGCCAACAACCAGTTCTCCCTGAAG12650.10533871601681423No Hit
CTCCTGCGGGGCCAGTCAGACTGTGACCAGCAGCTATTTAGCCTGGTACC12200.1015914889648327No Hit
GACTTCACTCTCACCATCAACAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATAGTAAATTA12130.1010085869789689No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)