FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1200888
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46180.3845487672455716No Hit
GTATTACTGTCAGCAGTATAGTAAATTACCGCTCACTTTCGGCGGAGGGA36180.3012770549793153No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31910.2657200338416239No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA24630.20509822731178928No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC23970.19960229430221635No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22100.18403048410842643No Hit
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGTTCAGCTTCAGGGAGA21560.1795338116460486No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21010.17495386747140448No Hit
GTGTATTACTGTGCGAGACTTGGCGGTTATTACGATGTTTTGACTGCTTA19960.1662103376834476No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19180.1597151441266796No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT19060.15871588357948452No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18690.15563483022563304No Hit
GTGTATTACTGTCAGCAGTATAGTAAATTACCGCTCACTTTCGGCGGAGGGACCAAGGTTCAGATCAAACGAA18470.1538028525557754No Hit
GTCAGGACCCAGAGGGAACCATGGATACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATTA17660.14705784386220863No Hit
GTCTGGGACAGACTTCACTCTCACCATCAACAGACTGGAGCCTGAAGATT16910.1408124654422394No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16480.1372317818147904No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16390.13648233640439408No Hit
GTCTTTGTATCCAGGGGAAAGAGCCACCCTCTCCTGCGGGGCCAGTCAGA15220.1267395460692421No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14350.11949490710207779No Hit
GTATAGTAAATTACCGCTCACTTTCGGCGGAGGGACCAAGGTTCAGATCAAACGAACTGTGGCTGCACCAT14330.11932836367754528No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGA14180.11807928799355144No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13930.11599749518689502No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13900.11574768005009625No Hit
GTGCAGCCACAGTTCGTTTGATCTGAACCTTGGTCCCTCCGCCGAAAGTG13640.1135826155311736No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA13450.11200045299811473No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG13160.10958557334239329No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13100.10908594306879575No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12950.10783686738480192No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12700.10575507457814551No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12500.1040896403328204No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12490.10400636862055414No Hit
GTCTCCAGCCACCCTGTCTTTGTATCCAGGGGAAAGAGCCACCCTCTCCTGCGGGGCCAGTCAGACTGTGACCAG12420.10342346663469032No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA12390.10317365149789155No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12380.1030903797856253No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12370.10300710807335904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT6400.046.31035670
GTGGTAT5800.046.185011
GGTATCA17350.045.724291
TGGTATC5800.044.9688572
GTATCAA48250.043.5600661
TATCAAC54950.037.9718862
ATCAACG59150.035.0201953
TCAACGC59900.034.660094
CAACGCA61500.033.8042145
AACGCAG62600.033.4262736
ATAGACG8700.031.7962370
CTACGGA6550.031.770169
CGCAGAG66800.031.3246238
AGAGTAC68000.031.0223611
GCAGAGT68950.030.5465349
CAGAGTA70750.029.96300110
CTATACC605.648662E-428.549774
ACGCAGA73600.028.477037
AGTACGG44450.027.50880813
GAGTACG45100.027.25855412
Produced by FastQC (version 0.11.5)