FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3298773
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT118030.35779970310172904No Hit
CTATTACTGCATGCAAGGTCTACAAAGTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGG82080.24881978844861408No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC75380.22850920630185828No Hit
CAGTAATAGACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA75090.22763009155222258No Hit
GTAATAGACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA73680.2233557750108904No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT72650.22023340193459812No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA72400.21947554439180872No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG72290.21914208707298136No Hit
GTAATAGACGGCCGTGTCTGCGGCGGTCACAAAGCGCAATGTCAGGGAGAACTGGTTCTTCGACGTGTCTACTGA70060.21238199779129996No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT69950.21204854047247265No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC65010.1970732754269542No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC64690.19610321777218379No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT63720.19316273050616092No Hit
GTGATGGATACAACGATTTGGCTTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGATT63350.19204110134283262No Hit
GTCTACAAAGTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA62170.1884640137408667No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA60200.18249209630368626No Hit
GTCTATTACTGCATGCAAGGTCTACAAAGTCCCACTTTCGGCGGAGGGAC59280.17970318054622128No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC56790.1721549194200389No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT56180.17030574701563278No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG55240.16745620265474467No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGTAGGTCTAGTCAGAGCCTCCTGCATAGTGATGGATACAACGATTTGG54930.1665164593016858No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT54160.16418225806989448No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG50550.1532387951520156No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC50080.15181402297157154No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAATCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA49840.15108647973049374No Hit
GTCTATTACTGTGCGAGAGATCGCACGTTCAGGGAGTACCCAAGAACCTA48840.14805504955933615No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG46460.14084024575198112No Hit
CTCCTGCATAGTGATGGATACAACGATTTGGCTTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGAT45690.13850604452018977No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG45620.13829384440820874No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT45230.13711158664145728No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGATTCTAATCGGGCC44850.1359596431764174No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGCTGACTCAGTCTCCACT44830.13589901457299428No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT44660.13538367144389749No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG44440.13471675680624282No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG43880.13301915591039457No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTGATGGATACAACGATTTGGCTTGGTACCTGCAGAAGCCAGGGCAGTCTC43470.13177626954021995No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGCTGACTCAGTCTCCACTCTCCCTGTCCGTCGCCC43150.13080621188544952No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA42770.12965426842040964No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA42670.1293511254032939No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT41970.1272291242834836No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41050.12444020852601861No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGCTGACTCAGTCTCCACTCTCC40400.12246977891476618No Hit
CTCCCTGTCCGTCGCCCCTGGAGAGCCGGCCTCCATCTCCTGTAGGTCTA39090.11849860539054975No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG38460.11658880438272048No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG38390.11637660427073945No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGCTGA38220.11586126114164266No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT38090.11546717521939219No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG38070.11540654661596902No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT37800.11458806046975648No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA37040.11228417353967672No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTCTATTACTGCATGCAAGGTCTACAAAGT36830.11164757320373364No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC36320.11010154381644326No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA36310.1100712295147317No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC36170.10964682929076963No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG36170.10964682929076963No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT35990.10910117185996125No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG35610.10794922839492138No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG35500.10761577107609405No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC35160.10658508481790048No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA35120.10646382761105416No Hit
CCTGTAGGTCTAGTCAGAGCCTCCTGCATAGTGATGGATACAACGATTTG34980.1060394273870921No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC34930.10588785587853423No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGATTCTAATCGGGCCTCCGGGGTCCCTGA34610.1049177982237638No Hit
GCATAGTGATGGATACAACGATTTGGCTTGGTACCTGCAGAAGCCAGGGC34250.10382648336214707No Hit
GTCTCCACTCTCCCTGTCCGTCGCCCCTGGAGAGCCGGCCTCCATCTCCT34110.10340208313818501No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA34000.10306862581935768No Hit
CCTGCATAGTGATGGATACAACGATTTGGCTTGGTACCTGCAGAAGCCAG33730.10225013967314514No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT33470.10146196782864415No Hit
GGTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCAC33370.10115882481152841No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC13750.053.07592
GTGGTAT14000.052.4161831
GGTATCA42650.043.135621
GTATCAA102800.040.01521
TATCAAC117050.034.997722
ATCAACG124050.032.9210973
TCAACGC125000.032.621594
CAACGCA127750.031.8813365
AACGCAG129200.031.5496586
CGCAGAG137550.029.634438
AGAGTAC139300.029.38550411
CAGAGTA141750.029.02308310
ACGCAGA143800.028.3942267
ACGCATA809.816918E-525.777087
AGTACGG112400.025.59555613
TATCGTA1001.0420264E-425.55157769
GAGTACG113600.025.47414612
CTACTGA29800.024.72272169
GGCCAAG32500.024.5890770
TCTACTG27000.024.3403368
Produced by FastQC (version 0.11.5)