FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences591685
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGCTTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22450.3794248628915724No Hit
ACCTTGAGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC19120.32314491663638595No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGCTTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18390.3108072707606243No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17780.3004977310562208No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17290.29221629752317535No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16990.2871460320947801No Hit
GAGTTACACTACCTTGAGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT16090.27193523580959467No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15670.26483686420984137No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15050.2543583156578247No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14250.24083760784877087No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12900.2180214134209926No Hit
CCTCAAGGTAGTGTAACTCTGTTGACAGTAATAAGTTGCAAAGTCTTCAG12060.2038246702214861No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG12050.20365566137387292No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG11700.1977403517074119No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11620.19638828092650648No Hit
GTGTAACTCTGTTGACAGTAATAAGTTGCAAAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAGT11390.19250107743140354No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAACGTC11220.18962792702197961No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10690.18067045809848145No Hit
ATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGTACCTTTTTAAATTGGTATCAGCAGAAA10650.17999442270802876No Hit
CCTGAAGACTTTGCAACTTATTACTGTCAACAGAGTTACACTACCTTGAG10070.17019190954646476No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9750.16478362642284322No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC9260.15650219288979778No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAAGCG9210.1556571486517319No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9000.1521079628518553No Hit
ATCTGAGACAGACTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGACT8960.1514319274614026No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8950.15126291861378943No Hit
ACTTATTACTGTCAACAGAGTTACACTACCTTGAGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAA8940.15109390976617626No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGCTTGGAGGGCGTTATCCACCTTCCACTGTA8670.1465306708806206No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8670.1465306708806206No Hit
GTCTCCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGTAC8660.14636166203300743No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8640.1460236443377811No Hit
ACACTACCTTGAGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGA8640.1460236443377811No Hit
GTCTGCAACCTGAAGACTTTGCAACTTATTACTGTCAACAGAGTTACACTACCTTGAGGACGTTCGGCCAGGGGA8600.1453476089473284No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8420.1423054496902913No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA8300.14027734351893323No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8290.14010833467132006No Hit
GTATTAGTACCTTTTTAAATTGGTATCAGCAGAAACCAGGGAGAGCCCCTAAGCTCCTGATCTTTGCTGCATCCA8250.13943229928086734No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC8250.13943229928086734No Hit
AGGTAGTGTAACTCTGTTGACAGTAATAAGTTGCAAAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAGT8190.1384182461951883No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG7960.13453104270008534No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7930.13402401615724582No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7830.1323339276811141No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT7720.1304748303573692No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7660.12946077727169017No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGCTTGGAGGGCGTTATCCA7560.12777068879555845No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7520.12709465340510576No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA7410.12523555608136086No Hit
GACTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGACTTTGCAACTTATTACTGTCAACAGAGTTACACTAC7360.12439051184329498No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7320.12371447645284231No Hit
GTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAGTCTGTCTCAGATCCACTGCCACTGAACCTTGATGGGAC7300.12337645875761596No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7280.12303844106238962No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7150.12084132604341838No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT7030.11881321987206031No Hit
CACCATCAGCAGTCTGCAACCTGAAGACTTTGCAACTTATTACTGTCAACAGAGTTACACTACCTTGAGGACGTT6900.11661610485308907No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT6900.11661610485308907No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6700.11323592790082561No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6680.11289791020559925No Hit
GTAATAAGTTGCAAAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAGTCTGTCTCAGATCCACTGCCA6680.11289791020559925No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG6630.1120528659675334No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6480.10951773325333582No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA6350.10732061823436458No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC6310.10664458284391189No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC6240.10546152091061967No Hit
GAGTATTAGTACCTTTTTAAATTGGTATCAGCAGAAACCAGGGAGAGCCCCTAAGCTCCTGATCTTTGCTGCATC6170.10427845897732746No Hit
GTTCAGTGGCAGTGGATCTGAGACAGACTTCACTCTCACCATCAGCAGTC6070.10258837050119575No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC6010.1015743174155167No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)