FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences591685
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23990.405452225424001No Hit
CTCCTGGGAGTTACCCGCTTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20150.3405528279405427No Hit
ACCTTGAGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC18810.31790564236037755No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGCTTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18000.3042159257037106No Hit
GAGTTACACTACCTTGAGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT15810.26720298807642584No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13790.23306320085856497No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13780.2328941920109518No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13060.22072555498280333No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12930.21852843996383212No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12090.2043316967643256No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11840.2001064755739963No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAACGTC11190.18912090047914007No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG11080.1872618031553952No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10870.18371261735551855No Hit
CCTCAAGGTAGTGTAACTCTGTTGACAGTAATAAGTTGCAAAGTCTTCAG10480.17712127229860483No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10410.17593821036531263No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10380.1754311838224731No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG10360.17509316612724676No Hit
GTGTAACTCTGTTGACAGTAATAAGTTGCAAAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAGT10090.1705299272416911No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGCTTGGAGGGCGTTATCCACCTTCCACTGTA9790.1654596618132959No Hit
ACACTACCTTGAGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGA9750.16478362642284322No Hit
CCTGAAGACTTTGCAACTTATTACTGTCAACAGAGTTACACTACCTTGAG9490.16038939638490074No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9390.15869930790876902No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9020.15244598054708164No Hit
ACTTATTACTGTCAACAGAGTTACACTACCTTGAGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAA8930.1509249009185631No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8710.1472067062710733No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAAGCG8620.14568562664255472No Hit
GTCTCCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGTAC8580.14500959125210205No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC8500.14365752047119665No Hit
ATCTGAGACAGACTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGACT8480.1433195027759703No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8270.1397703169760937No Hit
ATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGTACCTTTTTAAATTGGTATCAGCAGAAA8230.139094281585641No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGCTTGGAGGGCGTTATCCA8170.138080228499962No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8100.13689716656666975No Hit
GTCTGCAACCTGAAGACTTTGCAACTTATTACTGTCAACAGAGTTACACTACCTTGAGGACGTTCGGCCAGGGGA7960.13453104270008534No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7950.13436203385247217No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC7940.134193025004859No Hit
GTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAGTCTGTCTCAGATCCACTGCCACTGAACCTTGATGGGAC7880.13317897191918No Hit
GTATTAGTACCTTTTTAAATTGGTATCAGCAGAAACCAGGGAGAGCCCCTAAGCTCCTGATCTTTGCTGCATCCA7810.13199590998588775No Hit
GACTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGACTTTGCAACTTATTACTGTCAACAGAGTTACACTAC7370.12455952069090816No Hit
AGGTAGTGTAACTCTGTTGACAGTAATAAGTTGCAAAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAGT7350.12422150299568181No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7210.1218553791290974No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAAGCGGGTAACTCCCAGGAGAG6900.11661610485308907No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6850.1157710606150232No Hit
CACCATCAGCAGTCTGCAACCTGAAGACTTTGCAACTTATTACTGTCAACAGAGTTACACTACCTTGAGGACGTT6820.11526403407218368No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA6780.114587998681731No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6750.11408097213889147No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT6690.11306691905321244No Hit
GTAATAAGTTGCAAAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAGTCTGTCTCAGATCCACTGCCA6630.1120528659675334No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGAGACAGACTTCACT6590.11137683057708071No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6560.1108698040342412No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA6550.11070079518662801No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG6510.11002475979617532No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC6470.10934872440572264No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA6440.10884169786288313No Hit
GAGTATTAGTACCTTTTTAAATTGGTATCAGCAGAAACCAGGGAGAGCCCCTAAGCTCCTGATCTTTGCTGCATC6390.10799665362481725No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6360.10748962708197775No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT6160.10410945012971429No Hit
GTGGAAGGTGGATAACGCCCTCCAAGCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC6070.10258837050119575No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA6030.10191233511074305No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)