FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1523513
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48040.31532386005239205No Hit
GTAATAGACGGCCGTGTCTGCGGCGGTCACAAAGCGCAATGTCAGGGAGAACTGGTTCTTCGACGTGTCTACTGA45390.29792985028680424No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT35390.23229207758647283No Hit
GTCTATTACTGTGCGAGAGATCGCACGTTCAGGGAGTACCCAAGAACCTA32970.21640773659299264No Hit
CTATTACTGCATGCAAGGTCTACAAAGTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGG31700.20807173946005056No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30320.1990137268274048No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30120.1977009713733982No Hit
CAGTAATAGACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA29710.1950098226926846No Hit
GTAATAGACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA28600.18772402992294782No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28230.18529543233303555No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT27580.18102897710751403No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26800.17590923083688817No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT26490.17387445988317787No Hit
GTCTACAAAGTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA26390.17321808215617457No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26100.17131458674786496No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA25770.16914854024875403No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25610.16809833588554873No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT25360.16645739156804046No Hit
GTGATGGATACAACGATTTGGCTTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGATT24260.15923723657100397No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG24200.158843409934802No Hit
GTCTATTACTGCATGCAAGGTCTACAAAGTCCCACTTTCGGCGGAGGGAC23680.15543024575438477No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23000.1509668772107622No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC22760.14939157066595427No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA22650.14866955516625063No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA21570.14158067571461486No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGTAGGTCTAGTCAGAGCCTCCTGCATAGTGATGGATACAACGATTTGG21510.14118684907841286No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21490.14105557353301218No Hit
GTCATGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCAT21370.14026792026060822No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG21050.13816751153419762No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC20940.13744549603449396No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19650.12897822335615122No Hit
CTCCTGCATAGTGATGGATACAACGATTTGGCTTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGAT19000.12471176813062966No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAATCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA18680.12261135940421906No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG18320.12024839958700714No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG18240.11972329740540448No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18240.11972329740540448No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGATTCTAATCGGGCC17870.11729469981549222No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT17660.11591630658878527No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17640.1157850310433846No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTGATGGATACAACGATTTGGCTTGGTACCTGCAGAAGCCAGGGCAGTCTC17420.1143410000439773No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17220.11302824458997067No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGCTGACTCAGTCTCCACTCTCCCTGTCCGTCGCCC17080.11210931577216604No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA17080.11210931577216604No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17060.11197804022676537No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGACCTCCTGTG16960.11132166249976207No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16830.11046837145465775No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG16780.1101401825911561No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16520.10843360050094748No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTCTATTACTGCATGCAAGGTCTACAAAGT16460.10803977386474549No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16340.10725212059234152No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16300.10698956950154019No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGCTGACTCAGTCTCCACT16070.10547990072943257No Hit
ACTTACTACAATCCGTCCCTCAAGAGTCGAGTCACCATGTCAGTAGACAC16030.10521734963863125No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15890.1042984208208266No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA15850.10403586973002528No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGCTGACTCAGTCTCCACTCTCC15830.10390459418462461No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15820.10383895641192428No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT15810.10377331863922394No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA15800.10370768086652361No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15800.10370768086652361No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC4850.056.8491362
GTGGTAT5050.053.947141
GGTATCA15850.044.384731
GTATCAA41800.041.085231
ACGGGTA1250.035.7998316
TATCAAC47900.035.6879652
ATCAACG51150.033.0606773
TCAACGC51950.032.5291184
CAACGCA53100.032.0723085
AACGCAG54200.031.6753036
TTAAACG553.3037076E-431.2884224
ATAAACG450.00466202130.614233
AGAGTAC57950.029.74332611
CGCAGAG58000.029.7186648
CAGAGTA58300.029.56573510
CTACTGA16950.027.8210769
ACGCAGA62250.027.6344077
GCTTGTA2150.027.2673651
TTCGGGG18300.027.22511770
TGTCTAC15000.026.91532366
Produced by FastQC (version 0.11.5)