FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1377467
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48610.35289411651966984No Hit
GTATAATGTCTGGCCTCCGCCGTACACTTTTGGCCAGGGGACCAAGCTGG34850.2530006163487038No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32420.235359540373744No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31920.2317296893500897No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31300.22722867408075836No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT30290.21989637501297674No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29200.21198329978141037No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28300.20544956793883262No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC26970.19579416421591228No Hit
ACATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC26950.19564897017496607No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26940.19557637315449297No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATGTCTGG24780.17989541673230647No Hit
CTGGTATCCCAGCCCGGTTCATTGGCAGTGGGTCTGGGACAGAGTTCACT24150.17532180444250206No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23760.17249052064405174No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23300.16915105770228978No Hit
CATCTATGGTGCATCCATCAGGGCCACTGGTATCCCAGCCCGGTTCATTGGCAGTGGGTCTGGGACAGAGTTCA22870.1660293858219471No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21940.15927786291795012No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20050.14555702604853693No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA19740.14330651841387126No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCGA18530.13452227893662788No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18440.13386890575237012No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCGACAATGTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT18360.1332881295885854No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGCGGAGGCCAGACATTATA18310.13292514448622No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCGACAATGTAGCCTGGTA18310.13292514448622No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17920.13009386068776965No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17860.12965827856493115No Hit
GGCCAAAAGTGTACGGCGGAGGCCAGACATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGG17360.12602842754127686No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC16900.1226889645995149No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT16140.1171715910435604No Hit
ATGTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATC15940.11571965063409868No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGCGGAGG15930.1156470536136256No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15320.11121863536476737No Hit
GTTTATTACTGTCAGCAGTATAATGTCTGGCCTCCGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA15120.10976669495530564No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC15090.10954890389388638No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15080.10947630687341331No Hit
GTTCATTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAGCC15050.10925851581199404No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14960.10860514262773628No Hit
GTCAGAGTGTTAGCGACAATGTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTG14770.10722579923874766No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14590.10591905287023211No Hit
GTCCCAGACCCACTGCCAATGAACCGGGCTGGGATACCAGTGGCCCTGAT14470.10504788862455508No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14160.10279738098988941No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23900.044.9639131
GTATCAA62500.040.8156241
TATCAAC71300.035.6817632
ATCAACG74450.034.264293
TCAACGC75800.033.7446374
CAACGCA77200.033.1326835
AACGCAG78500.032.5839926
CGCAGAG83500.030.6684048
AGAGTAC86100.029.6625611
GCAGAGT86950.029.4120649
CAGAGTA90500.028.25833310
ACGCAGA92200.027.8117647
GTGGTAT11700.027.584151
GAGTACG57000.025.71531512
AGTACGG56900.025.7001813
GAGTACT44700.024.42071712
GCTTAGG1601.5208998E-424.16396770
TACGGGG24550.023.91281715
AGTACTT46100.023.6046313
CGGGTAG1653.3251126E-922.88980917
Produced by FastQC (version 0.11.5)