FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1377467
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44810.3253072487398972No Hit
GTATAATGTCTGGCCTCCGCCGTACACTTTTGGCCAGGGGACCAAGCTGG37660.2734003791016409No Hit
ACATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC27330.19840765695294335No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC27300.19818986589152407No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT27230.1976816867482125No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27190.19739129866632013No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26750.19419702976550438No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26480.19223691021273104No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26370.1914383429875271No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25670.1863565515544111No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATGTCTGG24130.1751766104015559No Hit
CTGGTATCCCAGCCCGGTTCATTGGCAGTGGGTCTGGGACAGAGTTCACT22840.16581159476052784No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22020.1598586390817348No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21520.15622878805808052No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA21300.15463165360767261No Hit
CATCTATGGTGCATCCATCAGGGCCACTGGTATCCCAGCCCGGTTCATTGGCAGTGGGTCTGGGACAGAGTTCA19920.1446132647823868No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19200.13938627930832462No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18090.13132801003581213No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17920.13009386068776965No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGCGGAGGCCAGACATTATA17830.1294404875035119No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCGACAATGTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT17700.12849672623736177No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17670.12827893517594252No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17070.12392311394755735No Hit
GGCCAAAAGTGTACGGCGGAGGCCAGACATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGG17060.12385051692708428No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16080.11673600892072188No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15750.11434030724511006No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15500.11252538173328291No Hit
GTTCATTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAGCC15450.11216239663091748No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15300.11107344132382119No Hit
GTCCCAGACCCACTGCCAATGAACCGGGCTGGGATACCAGTGGCCCTGAT15280.11092824728287502No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15230.11056526218050959No Hit
GTTTATTACTGTCAGCAGTATAATGTCTGGCCTCCGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA15120.10976669495530564No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14980.10875033666868245No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGCGGAGG14810.10751618732063999No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCGACAATGTAGCCTGGTA14780.10729839625922073No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14610.10606424691117827No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14410.10461230650171656No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT14240.1033781571536741No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13990.10156323164184695No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25200.045.1886021
GTATCAA68450.042.392431
CGGGCTA557.785535E-637.35542317
TATCAAC77400.037.024412
ATCAACG81050.035.2967953
TCAACGC81800.034.907184
CAACGCA83500.034.2238275
AACGCAG85100.033.658366
GATTTCG553.4036476E-431.1295217
CGCAGAG92150.031.0833028
GTGGTAT11050.031.0405271
AGAGTAC94700.030.35472911
GCAGAGT95800.030.113359
CAGAGTA98350.029.33257910
GTCTAGG605.6105957E-428.583151
ACGCAGA100950.028.3398137
TACGGGT16750.027.79678515
TAACCGC500.00797806527.42585424
GAGTACG62250.026.9372812
AGTACGG61950.026.792513
Produced by FastQC (version 0.11.5)