FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences841097
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11020.13101937113079706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15100.042.001351
GTATCAA39200.041.278551
GTGGTAT5550.037.679521
TATCAAC44500.036.593342
ATCAACG46300.035.096673
TCAACGC46800.034.72174
CAACGCA48650.033.542285
TGGTATC6150.033.4460332
AACGCAG49850.032.6660736
ACGCAGA53050.030.6883417
ATTCCGA800.004649255430.62704570
CGCAGAG53400.030.4230188
AGAGTAC54750.029.6728611
CAGAGTA55200.029.49305310
GCAGAGT56000.029.010529
CGTCAGA1800.028.5684855
GACGCGT1353.274181E-1027.95357926
CTCGTAT1306.179107E-926.58750539
AGACGCG1705.456968E-1226.22826425
GAGTACT33850.025.51567512
Produced by FastQC (version 0.11.5)