FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1358760
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49620.3651859047955489No Hit
CTATAACAGTTATCCGTGGACCTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAACTGTGGCTGCACCAT41650.3065294827636963No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC36420.2680385056963702No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35170.25883894138773583No Hit
CACTATCACCAGCCTGCAGCCTGATGATGTTGCAACTTATTACTGCCAACACTATAACAGTTATCCGTGGACCTT33190.24426683152285908No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32200.23698077659042066No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32100.23624481144572992No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGGTGGCTTTTT31840.234331302069534No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31360.23079866937501842No Hit
CTGTTATAGTGTTGGCAGTAATAAGTTGCAACATCATCAGGCTGCAGGCTGGTGATAGTGAGAGTGAATTCTGTC30350.22336542141364185No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28380.2088669080632341No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT27730.2040831346227443No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC27060.19915216815331624No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26160.19252848185109953No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG25820.190026200359151No Hit
CTCTAAGACTGTTCATTTGCAGATACAAGGTGTTCTTGGAATTGTCTCTG25620.1885542700697695No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTGTTAGTAAGTGGTTGGCCTGGTATCAGCAAAAA25510.1877447084106097No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24490.18023786393476404No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA24100.17736759987047013No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG22690.1669904913303306No Hit
CACTATAACAGTTATCCGTGGACCTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAACTGTGGCTGCACCAT22680.16691689481586153No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG22290.1640466307515676No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21770.16021961199917573No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21510.15830610262297978No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21500.1582325061085107No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT21320.15690776884806737No Hit
ACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACTATCACCAGC21270.156539786275722No Hit
GTGTTAGTAAGTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAATCTCCTGATATATAAGGCGTCTA20940.15411110129824251No Hit
GTTAGTAAGTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAA20120.1480761871117784No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT20090.14785539756837116No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT20060.14763460802496392No Hit
GTGCAGCCACAGTTCGTCTGATTTCCACCTTGGTCCCTTGGCCGAAGGTC20020.14734022196708763No Hit
GTCAGAGTGTTAGTAAGTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAATCTCCTGATATATAAGG19900.14645706379345874No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG19850.14608908122111336No Hit
GAGTGTTAGTAAGTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAATCTCCTGATATATAAGGCGT19800.145721098648768No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC19440.1430716241278813No Hit
CTCCTGTGAAGCCTCCGGATTCAGTTTTAAGGACTATGCCATGAATTGGGTCCGCCAGGCTCCAGGGGAGGGA19390.14270364155553592No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAATTGTGACAT19180.14115811475168535No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18970.1396125879478348No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18830.13858223674526776No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18590.13681592039800997No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGGTGGC18240.13424004239159232No Hit
GTATCAACGCAGAGTACGGGAGTCAGACCCAGTCAGGACACAGCATGGAC17730.13048662015366952No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT17560.12923547940769525No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC17050.12548205716977243No Hit
GAATTCACTCTCACTATCACCAGCCTGCAGCCTGATGATGTTGCAACTTATTACTGCCAACACTATAACAGTTAT16800.12364214430804557No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA16610.12224381053313316No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATTTCCACCTTGGTCCCTTGGCCGAAGGTCCACGGATAA16600.12217021401866408No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16560.12187582796078777No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGGTGGCTTTTTCTTGT16120.11863758132414849No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16080.1183431952662722No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15570.11458977302834937No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT15390.11326503576790602No Hit
TATATAAGGCGTCTACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT15340.11289705319556065No Hit
GATATATAAGGCGTCTACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA15000.11039477170361212No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14950.11002678913126673No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14810.10899643792869969No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14750.10855485884188525No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAATTGTGACATCCAGATGACCCAGTCTCCTTCCACC14680.10803968324060173No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14410.10605257734993671No Hit
GTGTCCCTTGGCCCCAGGTGTCAAAGGCATCGATTAGTGTTACTTCCCACCGCAAAACTTTCGCACAAAAATACA14280.10509582266183873No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14240.10480143660396243No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT14160.10421266448820984No Hit
GTTATAGTGTTGGCAGTAATAAGTTGCAACATCATCAGGCTGCAGGCTGG14140.10406547145927168No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14140.10406547145927168No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG14050.10340310282905No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA14020.1031823132856428No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13980.10288792722776649No Hit
CCTTATATATCAGGAGATTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCCAACCACTTACTAACACTCTGA13920.10244634814095205No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC13910.10237275162648296No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13820.10171038299626131No Hit
CCCTGAGACTCTCCTGTGAAGCCTCCGGATTCAGTTTTAAGGACTATGCC13800.10156318996732315No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13790.10148959345285408No Hit
CTCCAGGGGAGGGACTGGAGTGGGTCTCCGGTATCAGTGGTAGTGCTGGT13600.10009125967794165No Hit
CTCCAGTCCCTCCCCTGGAGCCTGGCGGACCCAATTCATGGCATAGTCCT13600.10009125967794165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4250.055.0975841
ATGCCGT1850.042.70110370
GTATCAA42200.040.2297251
TATCAAC46850.035.573972
TTCGATT400.002590590434.51603336
TACGACT400.002613166834.45501326
ATACGAC400.00261411134.45247325
TAATACG400.002616944734.44486223
ATCAACG49150.033.839213
TCAACGC49850.033.225884
TAACGGA11050.032.76649570
CAACGCA50900.032.6081245
AATAGAT2100.031.90685765
AACGCAG52350.031.7707186
ACGGGTT450.00467105430.60188315
ATAGATG2100.030.45601366
CGCAGAG55850.029.7720418
AGAGTAC56900.029.22264911
CAGAGTA57500.028.91771510
ACGCAGA57800.028.8282437
Produced by FastQC (version 0.11.5)