FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1358760
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47570.35009861932938857No Hit
CTGTTATAGTGTTGGCAGTAATAAGTTGCAACATCATCAGGCTGCAGGCTGGTGATAGTGAGAGTGAATTCTGTC39400.2899702670081545No Hit
CTATAACAGTTATCCGTGGACCTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAACTGTGGCTGCACCAT37220.2739262268538962No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT35320.259942889104772No Hit
CACTATCACCAGCCTGCAGCCTGATGATGTTGCAACTTATTACTGCCAACACTATAACAGTTATCCGTGGACCTT33530.2467691130148076No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31200.2296211251435132No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29900.22005357826253347No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC29240.21519620830757455No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28960.21313550590244043No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGGTGGCTTTTT28480.20960287320792487No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25720.18929023521446026No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT25320.18634637463569725No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25220.1856104094910065No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24190.1780299685006918No Hit
CTCTAAGACTGTTCATTTGCAGATACAAGGTGTTCTTGGAATTGTCTCTG24170.17788277547175366No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22690.1669904913303306No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA21600.15896847125320146No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21310.15683417233359828No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG20900.15381671524036622No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTGTTAGTAAGTGGTTGGCCTGGTATCAGCAAAAA20830.1533015396390827No Hit
GTGCAGCCACAGTTCGTCTGATTTCCACCTTGGTCCCTTGGCCGAAGGTC20820.1532279431246136No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20610.15168241632076304No Hit
CACTATAACAGTTATCCGTGGACCTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAACTGTGGCTGCACCAT20220.14881215225646915No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC20080.1477818010539021No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC19810.14579469516323706No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG19720.1451323265330154No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT19560.1439547823015102No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18560.13659513085460273No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA18320.13482881450734494No Hit
GTGTTAGTAAGTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAATCTCCTGATATATAAGGCGTCTA17950.13210574347198917No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17800.13100179575495305No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17770.1307810062115458No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17710.13033942712473137No Hit
GTTATAGTGTTGGCAGTAATAAGTTGCAACATCATCAGGCTGCAGGCTGG17700.1302658306102623No Hit
CTCCTGTGAAGCCTCCGGATTCAGTTTTAAGGACTATGCCATGAATTGGGTCCGCCAGGCTCCAGGGGAGGGA17670.13004504106685508No Hit
ACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACTATCACCAGC17320.12746916306043746No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGGTGGC17220.12673319791574672No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATTTCCACCTTGGTCCCTTGGCCGAAGGTCCACGGATAA17070.1256292501987106No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT16990.12504047808295798No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16890.12430451293826725No Hit
GAATTCACTCTCACTATCACCAGCCTGCAGCCTGATGATGTTGCAACTTATTACTGCCAACACTATAACAGTTAT16810.12371574082251464No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG16640.12246460007654038No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16640.12246460007654038No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16140.11878477435308664No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16040.11804880920839589No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT15830.11650328240454533No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAATTGTGACAT15780.11613529983219996No Hit
GTATCAACGCAGAGTACGGGAGTCAGACCCAGTCAGGACACAGCATGGAC15760.1159881068032618No Hit
GAGTGTTAGTAAGTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAATCTCCTGATATATAAGGCGT15740.11584091377432365No Hit
GTTAGTAAGTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAA15510.11414819394153493No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15390.11326503576790602No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA15380.11319143925343696No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15050.11076275427595748No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGGTGGCTTTTTCTTGT15010.1104683682180812No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14950.11002678913126673No Hit
ACTTATTACTGCCAACACTATAACAGTTATCCGTGGACCTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAA14800.10892284141423061No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14730.10840766581294711No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA14690.1081132797550708No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA14680.10803968324060173No Hit
GTCAGAGTGTTAGTAAGTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAATCTCCTGATATATAAGG14600.10745091112484913No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC14570.10723012158144189No Hit
CTCCAGTCCCTCCCCTGGAGCCTGGCGGACCCAATTCATGGCATAGTCCT14510.10678854249462745No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14440.10627336689334393No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA14340.10553740174865317No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14330.10546380523418411No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG14300.10524301569077689No Hit
GTGTTACTTCCCACCGCAAAACTTTCGCACAAAAATACACGGCCGTGTCC14290.1051694191763078No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14140.10406547145927168No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13690.10075362830816333No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT13660.10053283876475609No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)