FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1420455
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25280.17797114304923423No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21170.14903675230823926No Hit
CCCTTGGACAGGCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA19190.13509755676878182No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14300.10067196778497031No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT7700.046.9433941
GGTATCA24350.040.4336551
GTATCAA58600.039.3598181
TGGTATC9050.038.798262
TATCAAC63850.035.6909182
ATCAACG68350.033.5425723
TCAACGC68900.033.2748154
CAACGCA70900.032.336175
AACGCAG72300.031.8052486
ACGCAGA77450.029.6365367
CGCAGAG78350.029.2961048
AGAGTAC79200.028.76443511
CAGAGTA80750.028.21229710
GCAGAGT81650.028.1963529
TATATCG500.00781927527.5391545
AGTACGG52450.025.26028413
GAGTACG53950.024.5579612
GAGTACT42100.022.72404912
AGTACTT42800.021.95037313
TACGGGT9000.021.79722615
Produced by FastQC (version 0.11.5)