FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1420455
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24770.17438074419816185No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20080.14136315476379047No Hit
GTGTGTACCTTGCATGCAGTAATAAACCCCAGCATCCTCAGCCTCCACCC16560.11658236269364394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23600.044.1699521
GTGGTAT6550.043.594641
GTATCAA60750.040.4907721
TGGTATC7600.037.109862
TATCAAC67400.036.333662
TCAACGC70850.034.228484
ATCAACG71200.034.1856083
CAACGCA72050.033.6882825
AACGCAG73150.033.181696
ACGCAGA80050.030.3644437
CGCAGAG81100.029.844328
AGAGTAC82300.029.40916611
CAGAGTA84100.028.8205410
GCAGAGT85350.028.5593439
AGTACGG55050.025.6968113
GAGTACG55550.025.46282412
GAGTACT40700.024.29357712
ATCAGTG14850.024.07063970
AGGTACG1151.7439688E-623.9266152
AGTACTT42250.022.91720613
Produced by FastQC (version 0.11.5)