FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1082671
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40670.3756450482187109No Hit
GAGTTACACTACTCCTTATAGTCCTTCGGAGTACACTTTTGGCCAGGGGA39950.3689948285305508No Hit
GTGTAACTCTGTTGACAGTAGTAAGTCGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATTTGTC33030.3050788281943453No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG29580.27321319218857804No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27140.25067633657870214No Hit
GTGCATCCACTTTGCAAAATGGGGTCCCATCAAGGTTCAGTGGCAGTGGA25160.2323882324362618No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24070.22232053874168606No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23180.2141001282938215No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22860.21114447509908366No Hit
ATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCGTTAGGAC22850.21105211093674808No Hit
GTGGATGCACCGTAGATCAGGAGATTAGGGGCTTTCCCTAATTTCTGCTGATACCAATTCAAATAGGTCCTAACG22620.2089277352030303No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21910.2023698796772057No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21890.20218515135253462No Hit
GTGCAGCCACAGTTCGCTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG21380.1974745790734212No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18660.17235152691814964No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18340.1693958737234118No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG18130.16745622631436513No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17390.1606212783015339No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC17260.15942054419117163No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16810.15526415688607156No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT16070.14842920887324035No Hit
GTAGTAAGTCGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATTTGTCCCAGATCCACTGCCA15930.14713611060054255No Hit
ATAAGGAGTAGTGTAACTCTGTTGACAGTAGTAAGTCGCAAAATCTTCAG15810.14602774065251586No Hit
GATCTACGGTGCATCCACTTTGCAAAATGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAAATTTCA15700.14501173486682473No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14870.1373455093929735No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC14810.13679132441896014No Hit
GTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA14620.13503640533458458No Hit
ACTCCTTATAGTCCTTCGGAGTACACTTTTGGCCAGGGGACCAAGCTGGA14590.1347593128475779No Hit
ACTTACTACTGTCAACAGAGTTACACTACTCCTTATAGTCCTTCGGAGTA14550.13438985619823565No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14330.1323578446268534No Hit
GGCCAAAAGTGTACTCCGAAGGACTATAAGGAGTAGTGTAACTCTGTTGA14160.13078765386714894No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14100.13023346889313558No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13980.1291250989451089No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGAACTGTGGCTGCACCA13810.12755490818540444No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13750.12700072321139108No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13670.12626180991270664No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13540.12506107580234438No Hit
GTCTCCAGCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCGTTAGGAC13540.12506107580234438No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13430.12404507001665326No Hit
GTCTCTGGTGGCTCCATCAGCAATGATGGTTACTACTGGAGTTGGATCCG13170.12164360179592877No Hit
GGACTATAAGGAGTAGTGTAACTCTGTTGACAGTAGTAAGTCGCAAAATC13010.12016577519855987No Hit
CCGTAGATCAGGAGATTAGGGGCTTTCCCTAATTTCTGCTGATACCAATT12950.11961159022454651No Hit
CTATAAGGAGTAGTGTAACTCTGTTGACAGTAGTAAGTCGCAAAATCTTC12860.1187803127635265No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12830.11850322027651983No Hit
TTATAGTCCTTCGGAGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGAACTGTGGCTGCACCAT12730.11757957865316426No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCGACTTACTACTGTCAACAGAGTTACACTACTCCTTATAGTC12640.11674830119214423No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12270.11333082718572862No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT12140.11213009307536638No Hit
CCTTATAGTCCTTCGGAGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGAACTGTGGCTGCACCAT12110.11185300058835972No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCAGCTTGGTCC12030.11111408728967526No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11980.11065226647799747No Hit
GAGTAGTGTAACTCTGTTGACAGTAGTAAGTCGCAAAATCTTCAGGTTGC11960.11046753815332636No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11940.11028280982865524No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11730.10834316241960854No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11680.10788134160793075No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11660.10769661328325965No Hit
CTGCTGATGGTGAGAGTGAAATTTGTCCCAGATCCACTGCCACTGAACCT11550.10668060749756851No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCAGCCTCC11490.10612642252355517No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11280.10418677511450847No Hit
GTCTGCAACCTGAAGATTTTGCGACTTACTACTGTCAACAGAGTTACACTACTCCTTATAGTCCTTCGGAGTACA11050.10206239938079066No Hit
ACCTTGATGGGACCCCATTTTGCAAAGTGGATGCACCGTAGATCAGGAGATTAGGGGCTTTCCCTAATTTCTG11030.10187767105611954No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10950.10113875775743508No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10930.10095402943276396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4250.046.042641
GTATCAA35500.041.9696851
GGTTACC350.001394461439.156418
TATCAAC40100.037.049022
ATCAACG42350.035.1421623
TCAACGC42400.034.8387644
CAACGCA43250.034.1446085
AACGCAG44500.033.3379446
CGCAGAG46900.031.7050068
AGAGTAC47650.030.91836411
CGGACTT450.00445714630.89592645
AACGTTA450.004744176330.50429525
TCTAGAC450.004761378330.4817353
ACGCAGA48800.030.4705877
GCAGAGT48800.030.4003779
TGCTCGT704.0181072E-529.38766720
CAGAGTA51450.028.83456610
GCACGCG500.007540819227.74393742
CCGATTA500.00755976527.72967741
AGTACGG37350.027.61129813
Produced by FastQC (version 0.11.5)