FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1344728
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGTTTCAGGGAGA41620.30950497052191966No Hit
GTCTATTACTGTGCGAGGCAGTGGGAACTAAAGACATGGTTCGACCCCTGGGGCCAGGGAATCCTGGTCACCGT38030.28280812179117265No Hit
ACCTACTACTACGCGTCCCTCAAGAGCCGGCTCACCATTTCCGTCCACGCATCTAGGAATCAGGTCTCCCTGAAA36950.2747767578276053No Hit
GTATTACAGTACCCCTCTTACTTTCGGCGGAGGGACCAAGCTGGAGATCATACGAACTGTGGCTGCACCAT36000.26771213211891176No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30740.228596415037093No Hit
GTCTCTGGTGGCTCCATCAGCAGTACTGATTACTACTGGGCCTGGATCCG27400.2037586783349495No Hit
CTGTAATACTGTTGACAGTAATAGGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGTAATCCGAC26110.19416566026735518No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC22630.16828682082919372No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT22050.16397368092283346No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21070.15668596177070754No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20570.15296773771350045No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC20250.15058807431688787No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20170.14999315846773476No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT19720.14664675681624834No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19110.14211052346645567No Hit
GGTCTATGGTGCCTCCCTATTGGAAAGTGGGGTCCCATCCAGGTTCAGTGGCAGTGGATCGGGGTCGGATTACA18050.1342278884651766No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17960.13355860813487933No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTACTGATTACTACTGGG17610.13095585129483434No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17520.13028657096453708No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17440.12969165511538394No Hit
GTAGGAGACAGAGTCACCGTCACTTGCCGGGCGAGTCAGGGCATAAGCAATTCTTTAGCCTGGTATCAGCAGAAA17410.1294685616719515No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16910.1257503376147444No Hit
CTCTTATAGTGGGACGACCTACTACTACGCGTCCCTCAAGAGCCGGCTCA16220.12061918841579859No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15720.11690096435859147No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15550.11563676817914106No Hit
CAGCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTACCAGATGTGACAT15300.1137776561505375No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT15080.11214163756536638No Hit
CCATAGACCAGGAGACTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC15040.11184417964078981No Hit
GATTACTACTGGGCCTGGATCCGACAGCCCCCAGGGAAGGGCCTGGAGTGGATTGGCA14690.10924142280074484No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA14630.10879523591387998No Hit
TGTCAGGACACAGCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTC14530.10805159110243857No Hit
GTGCAGCCACAGTTCGTATGATCTCCAGCTTGGTCCCTCCGCCGAAAGTA14450.10745667525328542No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14310.10641557251726744No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13930.10358972223379002No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13740.10217679709205132No Hit
ACCTATTACTGTCAACAGTATTACAGTACCCCTCTTACTTTCGGCGGAGGGACCAAGCTGGAGATCATACGAA13620.10128442331832162No Hit
GTCAGGACACAGCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTCC13570.10091260091260092No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13480.10024332058230365No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCGC150.00219579770.233460
GACGGAA11300.060.13393870
TATAACG306.483898E-445.749612
GTATCAA36550.041.963221
GGACGGA12100.039.37281469
CTCGTAT2300.039.0842439
GTGGTAT5200.038.931161
CGTATGC2650.036.57405541
TATCAAC42850.035.5533752
ATCTCGT2450.035.25369637
TATGTCG400.0026669234.312215
ATCAACG45150.033.9702263
TCAACGC45650.033.5229874
CGAGACG3050.032.65751623
CAACGCA46900.032.629525
AACGCAG48350.031.863876
TATGCCG3050.031.82282643
CTTATAC3250.031.672811
GATGCGT13350.031.65956162
ACGAGAC3200.031.1220622
Produced by FastQC (version 0.11.5)