FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1344728
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGTTTCAGGGAGA40680.3025147092943703No Hit
GTATTACAGTACCCCTCTTACTTTCGGCGGAGGGACCAAGCTGGAGATCATACGAACTGTGGCTGCACCAT35550.2643657304674254No Hit
GTCTATTACTGTGCGAGGCAGTGGGAACTAAAGACATGGTTCGACCCCTGGGGCCAGGGAATCCTGGTCACCGT35210.26183733810852455No Hit
ACCTACTACTACGCGTCCCTCAAGAGCCGGCTCACCATTTCCGTCCACGCATCTAGGAATCAGGTCTCCCTGAAA32970.2451796943322367No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30170.2243576396118769No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29350.21825975215805724No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26320.19572731437138216No Hit
CTGTAATACTGTTGACAGTAATAGGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGTAATCCGAC24460.18189552087857175No Hit
GTCTCTGGTGGCTCCATCAGCAGTACTGATTACTACTGGGCCTGGATCCG23680.17609509134932863No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC22130.1645685967719866No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21500.15988363445990564No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT20140.14977006502430232No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19320.14367217757048265No Hit
GGTCTATGGTGCCTCCCTATTGGAAAGTGGGGTCCCATCCAGGTTCAGTGGCAGTGGATCGGGGTCGGATTACA18150.13497153327661804No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18000.13385606605945588No Hit
GTAGTAGGTCGTCCCACTATAAGAGACACTGCCAATCCACTCCAGGCCCTTCCCTGGGGGCTGTCGGATCCAGGC17560.13058402888911363No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17250.12827872997364523No Hit
GTAGGAGACAGAGTCACCGTCACTTGCCGGGCGAGTCAGGGCATAAGCAATTCTTTAGCCTGGTATCAGCAGAAA16850.12530415072787954No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16470.12247830044440214No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16280.12106537530266344No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16240.12076791737808687No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16120.11987554360435716No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15390.11444693648083477No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTACTGATTACTACTGGG14920.1109518058670601No Hit
CAGCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTACCAGATGTGACAT14660.10901832935731241No Hit
CTCTTATAGTGGGACGACCTACTACTACGCGTCCCTCAAGAGCCGGCTCA14530.10805159110243857No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14420.10723358180985298No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14360.10678739492298814No Hit
TGTCAGGACACAGCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTC14260.10604375011154672No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13990.10403590912065488No Hit
GTCAGGACACAGCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTCC13970.1038871801583666No Hit
GTGCAGCCACAGTTCGTATGATCTCCAGCTTGGTCCCTCCGCCGAAAGTA13910.10344099327150175No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA13760.10232552605433962No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13640.1014331522806099No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)