FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1360804
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19340.14212186325143078No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17700.13007016440280894No Hit
GTAATAGACCGCCGTGTCATCGGCTCTCAGTCTGTTCATTTGCAGATACA15780.11596085843369067No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA15380.11302141969012436No Hit
GGTGTATACTACGGAGCCTCTGTGAAGGGCCGTTTCACCAGCTCCAGGGACAACGCCAAGAACTCAGTGTATCTG14200.10435007539660378No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18750.045.7664871
GTATCAA48450.040.4732441
TATCAAC56000.035.2024962
ATCAACG59450.033.1016583
TCAACGC59950.032.825584
CAACGCA61100.032.207755
CGCTCGG1002.8679674E-432.04938570
AACGCAG61500.031.942236
ACGCAGA66900.029.461557
CGCAGAG67950.029.1584288
GCAGAGT71100.027.768659
TGGTATC7200.027.7626532
CGCGATT500.00778330627.5651112
CAGAGTA71900.027.4596810
AGAGTAC74850.026.46950111
AGTACGG47500.025.09875913
GAGTACG48000.024.83731312
GCGTTGT6250.024.66143262
GTGGTAT8250.024.6460651
CGTTGTC6850.023.0436963