FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2260115
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGCACTGGGAGCGGCGCCAACATCGGGGCAGGTTATGATGTACATTGGTACCAGCAACTTCCAGGAGCAGC74070.32772668647391834No Hit
GCCTTGGGCTGACCTAAGACGGTCAGCTTGGTCCCTCCGCCGAACACCACACTACTCAGGGTGTTGTCGTAGGA65160.2883039137389027No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC55480.24547423471814486No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT53010.23454558728206307No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG51000.22565223451019084No Hit
GACCTAAGACGGTCAGCTTGGTCCCTCCGCCGAACACCACACTACTCAGG49270.21799775675131577No Hit
ACTCAGGGTGTTGTCGTAGGACTGGCAGTAATAATCAGCCTCATCCTCAG48070.21268829240989948No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC45900.2030870110591718No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAAGACGGTCAGCTTGGTCCCTC42760.18919391269913258No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT41800.18494634122599957No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG40470.17906168491426322No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG38040.1683100196228953No Hit
GGTTATGATGTACATTGGTACCAGCAACTTCCAGGAGCAGCCCCCAAGCTCCTCATCTATGGGAACGCCAATCGC37470.16578802406072257No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36550.16171743473230343No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT35960.1591069480977738No Hit
TCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG35500.15707165343356422No Hit
GTCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC35250.1559655150291025No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCGGCGCCAACATCGGGGCAGGTTATGATGTACATTGGTACCAG34440.15238162659864654No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC33190.14685093457633794No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT32250.14269185417556185No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT31970.14145297916256472No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG30830.1364089880382193No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC30610.13543558624229296No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30150.13340029157808342No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA29960.1325596263906925No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA29740.13158622459476618No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG29370.12994913975616285No Hit
GAGTAGTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT28820.12751563526634707No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC28560.1263652513257069No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27650.12233890753346621No Hit
GTCCTACGACAACACCCTGAGTAGTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCTTAGGTCAGCCCAAGG26470.11711793426440689No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA25860.11441895655752031No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC25710.11375527351484328No Hit
CCCTCAGTGTCTGTGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGG25690.11366678244248633No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTAC25210.11154299670591984No Hit
GTCGTAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG25000.110613840446172No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC24740.1094634565055318No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT24280.10742816184132224No Hit
CCACACTACTCAGGGTGTTGTCGTAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGG24200.10707419755189447No Hit
ACACTACTCAGGGTGTTGTCGTAGGACTGGCAGTAATAATCAGCCTCATC23730.10499465735150645No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT23390.10349030912143853No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGG23140.1023841707169768No Hit
ACACCACACTACTCAGGGTGTTGTCGTAGGACTGGCAGTAATAATCAGCC23040.10194171535519211No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC22670.10030463051658876No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)