FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences770266
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14090.18292382112153463No Hit
GGTGTATACTATGGAGCCTCTGTGAAGGGCCGATTCACCAGCTCCAGGGACAACGCCAAGAACTCACTGTATCTG11100.1441060620616774No Hit
GTAATACACAGCCGTGTCATCGGCTCTCAGTCTGTTCATTTGCAGATACA10500.13631654519347863No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9990.12969545585550965No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9450.12268489067413074No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9330.121126987300491No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATGTTGCAGTTTATTACTGTCAGCAGCG9290.12060768617594442No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9200.1194392586457146No Hit
GTGTATTACTGTGTGAGAGATGGCGCCTTCGGATACAGTGGCGACGATCGTGAAGAGTACTGGGGCCAGGGAACC8970.11645327717957174No Hit
AGCCTGGAGAGTCCCTGACACTCTCCTGTGTAGCCTCTGGATTCGCCTTC8700.11294799458888229No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA8540.11087079009069595No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8020.10411987547159034No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7790.10113389400544746No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7730.1003549423186276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9500.043.539281
GTATCAA25200.042.1282841
TATCAAC27550.038.0071032
ATCAACG30250.034.7082823
TCAACGC30600.034.181024
CAACGCA31300.033.2915275
AACGCAG31950.032.823126
TGTACGG450.00468391530.583024
TACGGGT2150.030.39139215
CGCAGAG34800.029.937418
ACGCAGA35200.029.6948937
TGGTATC4450.028.6387832
CAGAGTA37500.027.96529810
GCAGAGT37300.027.9308829
GTCGAAC500.00780239127.54977628
GTGGTAT4950.027.1570781
AGAGTAC41000.025.9956411
GTAGTTT1551.00308265E-425.68729870
TGTTAGA2859.0039975E-1025.6121970
AGTACTT15450.024.70275313
Produced by FastQC (version 0.11.5)