FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1746392
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT59590.341217779284376No Hit
GTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA48880.27989134169189966No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT43810.2508600589100271No Hit
GTACACACTGGCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA43130.2469663168406635No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT39210.22452003902903817No Hit
GTGTGTACCTTGCATGCAGTAATAAAGCCCAACATCCTCAGCCTCCACCC38800.22217234160486304No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA35460.2030471967347537No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35260.20190197847905852No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32920.18850292488742504No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC32820.18793031575957747No Hit
GTGTATTACTGTGCGATAAGTCCTCTCGCTGCGCGTGACTACTGGGGCCA32670.1870714020678061No Hit
TTATTACTGCATGCAAGGTACACACTGGCCCTGGACGTTCGGCCAAGGGA32260.184723704643631No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31870.1824905290450254No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29970.17161095561592127No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29900.17121012922642798No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT29810.17069478101136515No Hit
GGTACACACTGGCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT28740.164567863343396No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT28540.16342264508770082No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC28160.16124673040188No Hit
GATCAGCACTGAGCACAGAGGACTCATTATGGAGTTGGGGCTGAGCTGGG27140.15540611729783463No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCGCTGATTTCACACTGCA26960.15437542086770897No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC26810.15351650717593757No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAATAGCTATTGGATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG26810.15351650717593757No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26010.14893563415315692No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC25990.1488211123275874No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA25660.14693150220569035No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25550.146301632165058No Hit
GTCTGGGACCCCAGAGTCCCGGTTAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGATA25270.1446983266070848No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25010.14320954287468107No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG24330.13931580080531747No Hit
AGGTACACACTGGCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT24160.13834236528797658No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA24140.13822784346240707No Hit
GGCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC23670.1355365805615234No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC23200.13284531766063978No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG22700.12998227202140183No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22090.12648935634153158No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT22030.12614579086482303No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC21860.1251723553474821No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21810.12488605078355833No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCATTATGGAGTTGGGGCTGAG21420.12265287518495274No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20760.11887365494115869No Hit
GTATCAACGCAGAGTACGGGAGGTCTCAGAGAGGAGCCTTAGCCCTGGAC20750.11881639402837392No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20690.11847282855166538No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20680.11841556763888061No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTTCATCCAATAGCTAT20670.11835830672609585No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC20660.1183010458133111No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGT20190.11560978291242745No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20160.11543800017407317No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT20050.11480813013344084No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19880.11383469461609994No Hit
CACTGGCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT19650.11251769362205048No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTAACCGGGAC19540.11188782358141816No Hit
AGTGTGTACCTTGCATGCAGTAATAAAGCCCAACATCCTCAGCCTCCACCCTGCTGATTTGCAGTGTGAAATCAG19460.11142973627914009No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA18970.10862395155268692No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA18940.10845216881433263No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18880.10810860333762408No Hit
GATTTCACACTGCAAATCAGCAGGGTGGAGGCTGAGGATGTTGGGCTTTATTACTGCATGCAAGGTACACACTGG18860.10799408151205457No Hit
GGGCTGGAGTGGGTGGCCAAGATAAAGGACGATGGAAGTGAAAAATACTA18840.10787955968648505No Hit
CTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCATT18360.10513103587281665No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT18330.10495925313446237No Hit
GCACTGAGCACAGAGGACTCATTATGGAGTTGGGGCTGAGCTGGGTTTTC18140.10387129579155195No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18140.10387129579155195No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACT18090.10358499122762815No Hit
GGGTTCCCTGGCCCCAGTAGTCACGCGCAGCGAGAGGACTTATCGCACAGTAATACACGGCCGTGTCCTCGGCT18040.10329868666370437No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17950.10278333844864154No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC17950.10278333844864154No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA17950.10278333844864154No Hit
CAGTAATAAAGCCCAACATCCTCAGCCTCCACCCTGCTGATTTGCAGTGTGAAATCAGCGCCTGACCCACTGCCG17830.10209620749522444No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTAAA17790.10186716384408541No Hit
GTCTCAGAGAGGAGCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTG17750.10163812019294637No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG17650.10106551106509878No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT17600.10077920650117499No Hit
GGTCTAGTCAAAGCCTCGTAAACCGTGATGGAAACACTTACTTGAATTGGTATCAGCAGAGGCCAGGCCAATCTC17490.10014933646054267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6300.053.0974851
GTATCAA49000.040.6090241
TATCAAC55400.035.901242
ATCAACG59550.033.3772933
TCAACGC59650.033.3022464
CAACGCA61600.032.29195
AACGCAG62400.031.8769916
CGCAGAG66100.030.0405868
CAGAGTA67550.029.34480310
AGAGTAC67100.029.33644911
GCAGAGT67800.029.2873579
ACGCAGA70000.028.4652217
TCTGGAG24350.027.80281370
GATCGTC500.00783088527.5311310
TTCGGGG16850.026.78530370
TGTTAGA11200.025.48226470
AGTACTT23200.025.2199813
GAGTACT23650.025.02758412
AGAGCAT6900.025.00989270
GTTATTA1601.2445047E-424.88965270
Produced by FastQC (version 0.11.5)